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Recombinant Human Tie-2 His-tag Protein, CF

Catalog # 10457-TI | R&D Systems, Inc. a Bio-Techne Brand
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10457-TI-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Tie-2 protein
Ala23-Lys745, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

81 kDa

SDS-PAGE

98-111 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Tie-2 His-tag (Catalog # 10457-TI) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Angiopoietin-2 His-tag  (Catalog # 623-AN) binds with an ED50 of 0.5-4 ng/mL.

Scientific Data Images for Recombinant Human Tie-2 His-tag Protein, CF

Recombinant Human Tie-2 His-tag Protein Binding Activity

Recombinant Human Tie-2 His-tag Protein Binding Activity

When Recombinant Human Tie-2 His-tag (10457-TI) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (623-AN) binds with an ED50 of 0.5-4 ng/mL.
Recombinant Human Tie-2 His-tag Protein SDS-PAGE

Recombinant Human Tie-2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Tie-2 His-tag Protein (Catalog # 10457-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 98-111 kDa.

Formulation, Preparation and Storage

10457-TI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-2 cDNA encodes a 1124 amino acid (aa) precursor protein with an 18 aa putative signal peptide, a 727 aa extracellular domain (ECD) and a 354 aa cytoplasmic domain. Human Tie-2 ECD shares 90% aa identity with mouse and rat Tie-2. Two ligands, angiopoietin1 (Ang1) and angiopoietin2 (Ang2), which bind Tie-2 with high affinity, have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to over-express Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.

References

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Takakura, N. et al. (1998) Immunity 9:677.
  3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Gene Symbol

TEK

UniProt

Product Documents for Recombinant Human Tie-2 His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Tie-2 His-tag Protein, CF

For research use only

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