Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

Name: Recombinant Human SIRP alpha/CD172a His-tag Protein, CF
Brand: R&D Systems
SKU: 9378-SA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9378-SA

Citations (2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P78324.2
Applications:	Bioactivity

View Full Technical Details for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

Product Specifications for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Gly27 & Glu31-Arg370, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly27 & Glu31
Predicted Molecular Mass	38 kDa
SDS-PAGE	44-63 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha /CD172a His-tag is coated at 0.5 μg/mL, 100 μL/well, Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) binds with an ED₅₀ of 0.04-0.24 μg/mL.

Scientific Data Examples for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

	Recombinant Human SIRP alpha/CD172a His-tag Protein Bioactivity When Recombinant Human SIRP alpha/CD172a (Catalog # 9378-SA) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) binds with an ED₅₀ of 0.04-0.24 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9378-SA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SIRP alpha/CD172a

Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1-4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha 2 or PTPNS) (5). One reported isoform lacks aa 1-101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma, respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3-6). It is also found on neurons, smooth muscle and endothelial cells (7-9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).

Item	Value
References	Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781. Liu, Y. et al. (2005) J. Biol. Chem. 280:36132. Kharitonenkov, A. et al. (1997) Nature 386:181. Swissprot Accession # P7832. Miyashita, M. et al. (2004) Mol. Biol. Cell 15:3950. Wang, X.X. & K.H. Pfenninger (2005) J. Cell Sci. 119:172. Maile, L.A. et al. (2003) Mol. Biol. Cell 14:3519. Johansen, M.L. & E.J. Brown (2007) J. Biol. Chem. 282:24219. Takenaka, K. et al. (2007) Nat. Immunol. 8:1313. Ishikawa-Sekigami, T. et al. (2006) Biochem. Biophys. Res. Commun. 343:1197. Olsson, M. et al. (2005) Blood 105:3577. Ide, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:5062. Gardai, S.J. et al. (2003) Cell 115:13. Lundberg, P. et al. (2007) Biochem. Biophys. Res. Commun. 352:444.
Long Name	Signal-regulatory Protein alpha
Entrez Gene IDs	140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)
Alternate Names	BIT, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD-1, MYD1, Macrophage fusion receptor, MyD-1 antigen, P84, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SHPS1CD172A, SIRP alpha, SIRPA, SIRPalpha, SIRPalpha2, SIRPtyrosine-protein phosphatase non-receptor type substrate 1, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-1, Sirp-alpha-2, Sirp-alpha-3, protein tyrosine phosphatase, non-receptor type substrate 1, signal-regulatory protein alpha

Citations for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF (2)

Showing 1 - 2 of 2 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF include:

Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Standard

YV Vladimirov et al. (2022), A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPalpha) Biomolecules, 12(7).
PMID: 35883493
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

PE van Bommel et al. (2018), CD20-selective inhibition of CD47-SIRP? don't eat me signaling with a bispecific antibody-derivative enhances the anticancer activity of daratumumab, alemtuzumab and obinutuzumab Oncoimmunology, 7(2):e1386361.
PMID: 29308308

There are no citations that match your criteria.

Showing 1 - 2 of 2 citations Showing All

Product Documents for Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS