Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

Name: Recombinant Human Semaphorin 6A Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1146-S6
Price: 428 USD

Catalog # 1146-S6 | R&D Systems, Inc. a Bio-Techne Brand

Citations (6)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9H2E6
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

Product Specifications for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 6A protein

Human Semaphorin 6A (Gly19-Thr649) Accession # Q9H2E6	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly19

Predicted Molecular Mass

97 kDa (monomer)

SDS-PAGE

120 kDa, reducing conditions

Activity

Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED₅₀ for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1146-S6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta propeller N-terminal semaphorin (sema) domain and also an extracellular
plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, human Sema6A shares 94% and 93% aa sequence identity with mouse and rat Sema6A, respectively. Alternative splicing generates an additional isoform with a 17 aa insertion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4
(3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).

Item	Value
References	Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9. Suto, F. et al. (2005) J. Neurosci. 25:3628. Matsuoka, R.L. et al. (2011) Nature 470:259. Runker, A.E. et al. (2008) Neural Dev. 3:34. Suto, F. et al. (2007) Neuron 53:535. Haklai-Topper, L. et al. (2010) EMBO J. 29:2635. Xu, X.M. et al. (2000) J. Neurosci. 20:2638. Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516. Mauti, O. et al. (2007) Neural Dev. 2:28. Segarra, M. et al. (2012) Blood 120:4104. Kigel, B. et al. (2011) Blood 118:4285. Bernard, F. et al. (2012) Glia 60:1590. Gautier, G. et al. (2006) Am. J. Pathol. 168:453.
Entrez Gene IDs	57556 (Human); 20358 (Mouse)
Alternate Names	KIAA1368HT018, SEMA, SEMA6A-1, SEMA6A1, SEMAQ, Sema VIA, Sema6A, Semaphorin 6A, Semaphorin VIA, VIA, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A, semaphorin 6A-1, semaphorin-6A, semaphorin-6A-1

Citations for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Semaphorin 6A Fc Chimera Protein, CF include:

Species: Human
Sample Types: Protein
Applications: Reference Standard

S Tian et al. (2022-11-09), Identification of TFPI as a receptor reveals recombination-driven receptor switching in Clostridioides difficile toxin B variants Nature Communications, 2022-113(1):6786.
PMID: 36351897
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Tian et al. (2017-01-13), Neurotrophin Promotes Neurite Outgrowth by Inhibiting Rif GTPase Activation Downstream of MAPKs and PI3K Signaling Int J Mol Sci, 2017-018(1).
PMID: 28098758
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Bioassay

Ebert A et al. (2014-06-01), Sema6a and Plxna2 mediate spatially regulated repulsion within the developing eye to promote eye vesicle cohesion. Development, 2014-0141(12):2473-82.
PMID: 24917502
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Segarra et al. (2012-09-24), Semaphorin 6A regulates angiogenesis by modulating VEGF signaling. Blood, 2012-0120(19):4104-15.
PMID: 23007403
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tran-Van H et al. (2011-01-01), Measles virus modulates dendritic cell/T-cell communication at the level of plexinA1/neuropilin-1 recruitment and activity. Eur. J. Immunol., 2011-041(1):151-63.
PMID: 21182086
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Renaud J et al. (2008-03-09), Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells. Nat. Neurosci., 2008-011(4):440-9.
PMID: 18327254

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Showing 1 - 6 of 6 citations Showing All

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Recombinant Human Semaphorin 6A Fc Chimera Protein, CF

Catalog # 1146-S6 | R&D Systems, Inc. a Bio-Techne Brand

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