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Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

Catalog # 10804-S3 | R&D Systems, Inc. a Bio-Techne Brand
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10804-S3-025

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3G protein
Human Semaphorin 3G
(Gly23-Thr782)(R557S, R558S, R560S, R561S, R774S, R777S)
Accession # Q9NS98.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly23

Predicted Molecular Mass

111 kDa

SDS-PAGE

105-125 kDa, under reducing conditions

Activity

Measured by the ability to inhibit the proliferation of HUVECumbilical vein endothelial cells.
The ED50 for this effect is 100-600 ng/mL.

Scientific Data Images for Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

Recombinant Human Semaphorin 3G Fc Chimera Protein Bioactivity.

Recombinant Human Semaphorin 3G Fc Chimera inhibits the proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 100-600 ng/mL.

Formulation, Preparation and Storage

10804-S3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3G

Semaphorin 3G (Sema3G), also known as Semaphorin sem2, is a class 3 member of the semaphorin family initially identified as regulators of neuron-axonal guidance (1, 2). More than 30 semaphorins have been discovered and are divided into eight classes: classes 1 and 4–7 are membrane-associated, while classes 2, 3, and 8 are in a secreted form (1, 2). Mature human Sema3G consists of an N-terminal sema domain, a plexin-semaphorin-integrin (PSI) domain, an Ig-like C2-type domain and a C-terminal basic domain (3). Within the extracellular domain, human Sema3G shares 87% amino acid sequence identity with mouse and rat Sema3G. In vitro analysis shows Sema 3G binds to Neuropilin-2, which forms a receptor complex with Plexin-D1. Via this pathways Sema 3G controls lymphatic vascular patterning by the means of cellular collapse (4). It shows protective effects in ischemic retinopathies by coordinating interactions of beta-catenin and VE-Cadherin in endothelium (5). Sema 3G has been shown to be involved in adipocyte differentiation. Knockdown of Sema3G inhibited weight gain through PI3K/Akt/GSK3 beta signaling in the adipose tissue and the AMPK/SREBP-1c pathway in the liver. Thus, Sema 3G is an adipokine essential for adipogenesis, lipogenesis, and insulin resistance and is associated with obesity (6).

References

  1. Alto, L.T. and Terman, J.R. (2017) Methods Mol. Biol. 1493:1.
  2. Jackson, R.E. and Eickholt, B.J. (2009) Curr. Biol. 19:R504.
  3. Toledano S. et al. (2019) J. Mol. Sci. 20:556.
  4. Liu, X. et al. (2016) Cell Rep. 17:2299.
  5. Chen, D. et al. (2021) J. Clin. Invest. 131:e135296.
  6. Liu, M. et al. (2020) J. Endocrinol. 244:223.

Alternate Names

sem2, SEMA3G, Semaphorin-3G

Entrez Gene IDs

56920 (Human); 218877 (Mouse); 290562 (Rat)

Gene Symbol

SEMA3G

UniProt

Product Documents for Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

For research use only

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