Recombinant Human PDGF-AB Protein, CF Best Seller

Name: Recombinant Human PDGF-AB Protein, CF
Brand: R&D Systems
SKU: 222-AB
Rating: 4 (1 reviews)

Catalog # 222-AB | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (11)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P04085.1 (A chain) & P01127.1 (B chain)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human PDGF-AB Protein, CF

Technical Data
Citations (11) / Reviews (1)

Product Specifications for Recombinant Human PDGF-AB Protein, CF

Source

E. coli-derived human PDGF-AB protein

Human PDGF-A
(Ser87 - Thr211)
Accession # P04085

Human PDGF-B (Ser82 - Thr190) Accession # P01127
N-terminus	C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser87 (A chain) & Ser82 (B chain)

Predicted Molecular Mass

14.3 kDa (A chain) & 12.3 kDa (B chain)

SDS-PAGE

14.3 kDa (A chain) & 12.3 kDa (B chain)

Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 15-75 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

222-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl. Reconstitution Buffer Available: Size / Price Qty Loading... RB03:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PDGF-AB

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Item	Value
Long Name	Platelet-derived Growth Factor AB
Alternate Names	PDGF-AB, PDGFAB

Citations for Recombinant Human PDGF-AB Protein, CF (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PDGF-AB Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Madsen et al. (2023-06-09), Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors Scientific reports
PMID: 37296166
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MR Hadjichara et al. (2019-04-15), Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosis Sci Rep, 2019-09(1):6020.
PMID: 30988425
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

SB Mamer et al. (2017-11-27), Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017-17(1):16439.
PMID: 29180757
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H He et al. (2017-03-02), Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 2017-07(0):43736.
PMID: 28252047
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hayes B et al. (2014-03-25), Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis. PLoS ONE, 2014-09(3):e92925.
PMID: 24667490
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ding W et al. (2010-07-06), Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood, 2010-0116(16):2984-93.
PMID: 20606160
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ball SG et al. (2010-03-15), Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells. Biochem. J., 2010-0427(1):29-40.
PMID: 20102335
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Li R et al. (2007-12-01), PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Invest. Ophthalmol. Vis. Sci., 2007-148(12):5722-32.
PMID: 18055825
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Gupta S et al. (2007-06-01), Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice. Cell, 2007-0129(5):957-68.
PMID: 17540175
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Rhodes KE et al. (2006-04-21), The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 2006-0140(1):87-100.
PMID: 16631314
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nagineni CN et al. (2005-04-01), Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 2005-0203(1):35-43.
PMID: 15368539

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Customer Reviews for Recombinant Human PDGF-AB Protein, CF (1)

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Recombinant Human PDGF-AB Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 12/15/2017

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Product Documents for Recombinant Human PDGF-AB Protein, CF

Product Datasheets

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COA

SDS

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