Recombinant Human PD-1 His Tagged Protein, CF
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Source: | HEK293 |
Accession #: | Q15116.3 |
Applications: | Bioactivity |
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Citations for Recombinant Human PD-1 His Tagged Protein, CF (7)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PD-1 His Tagged Protein, CF include:
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Species: Mouse
Sample Types: In Vivo
Applications: In VivoT Phakham et al. (2022), Highly efficient hybridoma generation and screening strategy for anti-PD-1 monoclonal antibody development Scientific Reports, 12(1):17792.
PMID: 36273231 -
Species: Human
Applications: BioassayCE Kang et al. (2022), Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platform Scientific Reports, 12(1):19030.
PMID: 36347901 -
Species: Human
Sample Types: Protein
Applications: BioassayH Issafras et al. (2021), Structural basis of HLX10 PD-1 receptor recognition, a promising anti-PD-1 antibody clinical candidate for cancer immunotherapy PLoS ONE, 16(12):e0257972.
PMID: 34972111 -
Species: Human
Sample Types: Protein
Applications: BioassayCJ Edwards et al. (2021), The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity British Journal of Cancer, 0(0).
PMID: 34969998 -
Species: Human
Sample Types: Serum
Applications: BioassayM Metz et al. (2021), Fenebrutinib in H1 antihistamine-refractory chronic spontaneous urticaria: a randomized phase 2 trial Nature Medicine, 27(11):1961-1969.
PMID: 34750553 -
Species: Human
Sample Types: Protein
Applications: Surface Plasmon Resonance (SPRM Crauwels et al. (2020), Reshaping Nanobodies for affinity purification on protein A N Biotechnol, 0(0).
PMID: 32001339 -
Species: Human
Sample Types: Reference Standard
Applications: BioassayME Brown et al. (2020), Assessing the binding properties of the anti-PD-1 antibody landscape using label-free biosensors PLoS ONE, 15(3):e0229206.
PMID: 32134960
There are no citations that match your criteria.
Customer Reviews for Recombinant Human PD-1 His Tagged Protein, CF (10)
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Application: SDS-PAGE ControlVerified Customer | Posted 10/15/2020
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Application: Binding assay/Protein-protein interactionReason for Rating: Good binding of PD-1-his tag with PDL1-Fc by OctetVerified Customer | Posted 06/19/2020
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Application: Immunoassay StandardVerified Customer | Posted 02/08/2019
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Application: SDS-PAGE ControlVerified Customer | Posted 01/30/2019
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Application: Cell migration/motilityVerified Customer | Posted 12/06/2018
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Application: CellProlifVerified Customer | Posted 10/24/2018
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Application: Immunoassay StandardVerified Customer | Posted 10/12/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 08/29/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/30/2017
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Application: Binding assay/Protein-protein interactionReason for Rating: This protein was used for a multitude of assays, including mouse immunizations, cell sorting, and Octet/binding assays (image of this assay included). The protein has performed well for all assays tested.Verified Customer | Posted 05/17/2017An anti-PD1 antibody was tested for binding to PD-1-His using ForteBio Octet, and the antibody bound specifically.
There are no reviews that match your criteria.
FAQs for Recombinant Human PD-1 His Tagged Protein, CF
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The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?
Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.
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What is the recommended method for reconstitution of a lyophilized protein or antibody?
Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand. Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.
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Are R&D Systems recombinant proteins and antibodies sterile?
Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.