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Recombinant Human P-Selectin/CD62P His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11035-PS

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11035-PS-100

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human P-Selectin/CD62P protein
Trp42-Ala771, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

N-terminal Sequence Analysis

Trp42

Predicted Molecular Mass

81 kDa

SDS-PAGE

105-125 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
The ED50 for this effect is 0.200-1.00 μg/mL.

Scientific Data Images for Recombinant Human P-Selectin/CD62P His-tag Protein, CF

Recombinant Human P-Selectin/CD62P His-tag Protein Bioactivity.

Immobilized Recombinant Human P-Selectin/CD62P His-tag Protein (Catalog # 11035-PS) supports the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 0.200-1.00 ug/mL.

Recombinant Human P-Selectin/CD62P His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human P-Selectin/CD62P His-tag Protein (Catalog # 11035-PS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125- kDa.

Formulation, Preparation and Storage

11035-PS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: P-Selectin/CD62P

P-Selectin, also known as GMP-140, PADGEM, and CD62P, is a cell surface glycoprotein of the Selectin family and is expressed by activated platelets and endothelial cells (1 - 3). In humans, there are 3 Selectins, P, E, and L and they are Ca2+-dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (4). Mature human P-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains, a transmembrane domain and a short cytoplasmic domain (5). The mature ECD of human P-selectin shares 66% amino acid sequence identity with mouse P-Selectin. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides (6). P-Selectin binds to P-Selectin glycoprotein ligand-1 (PSGL-1), a dimeric molecule rich in O- and N-glycans, present on myeloid cells, neutrophils, monocytes and lymphocytes (7). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium (8). This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (1, 3).

References

  1. Kansas, G.S. (1996) Blood 88:3259.
  2. Lü, S. (2015) PLOS One 10:1.
  3. Edwards, E.E. (2017) Integr. Biol. (Camb) 9:313.
  4. McEver, R.P. (1997) J. Clin. Invest. 100:485.
  5. Patel, K.D. (1995) J. Cell Biol. 131:1893.
  6. Zhang, N. (2016) Arterioscler Thromb. Vasc. Biol 36:1114.
  7. Frenette, P.S. et al. (2000) J Exp. Med. 191:1413.
  8. Zuchtriegel, G. (2016) PLOS Biology 14:1.

Alternate Names

CD62P, GMP140, GRMP, PADGEM, PSEL, SELP

Entrez Gene IDs

6403 (Human); 20344 (Mouse)

Gene Symbol

SELP

UniProt

Additional P-Selectin/CD62P Products

Product Documents for Recombinant Human P-Selectin/CD62P His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human P-Selectin/CD62P His-tag Protein, CF

For research use only

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