Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10842

Biotinylated
Discontinued Product
AVI10842 has been discontinued. View all OX40 products.

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein (AVI10842)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession #

P43489.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all OX40 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human OX40/TNFRSF4 protein
Human OX40/TNFRSF4
(Leu29-Ala216)
Accession # P43489.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu29

Predicted Molecular Mass

49 kDa

SDS-PAGE

64-72 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) is immobilized at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag binds with an ED50 of 3.00-15.0 ng/mL.

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Scientific Data Images for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human OX40 Ligand/TNFSF4 (1054-OX) is immobilized at 0.500 μg/mL (100 μL/well), Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag (Catalog # AVI10842) binds with an ED50 of 3.00-15.0 ng/mL.

Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human OX40/TNFRSF4 Fc Chimera Avi-tag Protein (Catalog # AVI10842) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-72 kDa and 128-144kDa, respectively.

Formulation, Preparation and Storage

AVI10842
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells' immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18). Our Avi-tag Biotinylated human OX40/TNFRSF4 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Hori, T. (2006) Int. J. Hematol. 83:17.
  2. Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
  3. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  4. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
  5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
  6. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  7. Xiao, X. et al. (2008) J. Immunol. 181:3193.
  8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
  9. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
  10. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  11. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
  12. Griseri, T. et al. (2010) J. Exp. Med. 207:699.
  13. Xiao, X. et al. (2012) J. Immunol. 188:892.
  14. Vu, M.D. et al. (2007) Blood 110:2501.
  15. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
  16. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
  17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
  18. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
  19. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757.

Alternate Names

ACT-135, CD134, Ly-70, TNFRSF4, Txgp1, TXGP1L

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Gene Symbol

TNFRSF4

UniProt

P43489.1

Additional OX40 Products

Product Documents for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human OX40/TNFRSF4 Fc Avi-tag Protein, CF

For research use only

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