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Recombinant Human Neurturin Protein

Bio-Techne includes R&D Systems | Catalog # 1297-NE

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Carrier Free
1297-NE-025/CF

Catalog #
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Size / Price
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With Carrier
1297-NE-025

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity, Binding Activity

Product Specifications

Source

E. coli-derived human Neurturin protein
Ala96-Val197, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

11.8 kDa (monomer)

Activity

Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The ED50 for this effect is typically 20-80 ng/mL in the presence of Recombinant Human GFR alpha‑2/GDNF R alpha‑2 Fc Chimera (Catalog # 613‑FR).

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Human GFR alpha‑2/GDNF R alpha‑2 Fc Chimera (Catalog # 613-FR) at 1 μg/mL can bind Recombinant Human Neurturin with an apparent KD <3.0 nM.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1297-NE
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1297-NE/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta) superfamily (1-4). Similarly to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Mature human Neurturin shares approximately 92% amino acid sequence identity with mouse Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2-5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha-1-GFR alpha-4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha-2 but can also bind GFR alpha-1 at higher concentrations (5‑8). Neurturin had been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non-neuronal systems (9).

References

  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  3. Milbrandt, J. et al. (1998) Neuron 20:245.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J. Biol. Chem. 272:33111.
  9. Airaksinen. M. and M. Saarma (2002) Nature Review Neuroscience 3:383.

Alternate Names

NRTN, NTN

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Gene Symbol

NRTN

UniProt

Additional Neurturin Products

Product Documents for Recombinant Human Neurturin Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Neurturin Protein

For research use only

Citations for Recombinant Human Neurturin Protein (9)

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Reconstitution Calculator

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