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Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

Catalog # 10093-MS | R&D Systems, Inc. a Bio-Techne Brand
N-Terminal/MPF
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10093-MS-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Mesothelin protein
Arg35-Leu289, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg35

Predicted Molecular Mass

28 kDa

SDS-PAGE

27-39 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Osteopontin (Catalog # 1433-OP/CF) is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog # 10093-MS) that produces 50% of the optimal binding response is 3-18 ng/mL.

Scientific Data Images for Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

Recombinant Human Mesothelin (aa 35-289) His-tag Protein Binding Activity

Recombinant Human Mesothelin (aa 35-289) His-tag Protein Binding Activity

When Recombinant Human Osteopontin (OPN) Protein (Catalog # 1433-OP/CF) is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin (aa 35-289) His-tag Protein (Catalog # 10093-MS) that produces 50% of the optimal binding response is 0.003-0.018 µg/mL.
Recombinant Human Mesothelin (aa 35-289) His-tag Protein SDS-PAGE

Recombinant Human Mesothelin (aa 35-289) His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-39 kDa.

Formulation, Preparation and Storage

10093-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Megakaryocyte-Potentiating Factor (MPF), is derived from a 70 kDa Mesothelin precursor (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic furin cleavage site between aa 288-293. The cleavage releases soluble N-terminal MPF (aa 37-286) and C-terminal Mesothelin (aa 296-606) remains attached to the cell surface via a GPI linkage. (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). This recombinant human MPF shares 56% sequence identity with mouse and rat MPF. Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).

References

  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

Product Documents for Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

For research use only

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