Recombinant Human Matrilin-3 Protein, CF

Name: Recombinant Human Matrilin-3 Protein, CF
Brand: R&D Systems
SKU: 3017-MN
Price: 476 USD

Catalog # 3017-MN | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	O15232
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Matrilin-3 Protein, CF

Product Specifications for Recombinant Human Matrilin-3 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Matrilin-3 protein Asp29-Arg486, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asp29
Predicted Molecular Mass	50.6 kDa
SDS-PAGE	57 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. Immobilized rhCOMP at 2 µg/mL (100 µL/well) can bind rhMatrilin-3 with a linear range of 1.5-100 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3017-MN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Matrilin-3

Matrilin-3 is a 50 - 60 kDa extracellular matrix protein that belongs to the superfamily of von Willebrand factor A (VWA) containing proteins. It is primarily expressed in cartilage and functions as a bridging component between proteins of the collagenous matrix (1 - 3). The human Matrilin-3 cDNA encodes a 486 amino acid (aa) precursor with a 28 aa signal sequence, an N-terminal VWA domain, four tandem EGF-like repeats, and a C-terminal coiled coil domain (4). The Matrilins differ in the number of VWA domains (one or two) and EGF-like repeats (one, three, four, or ten) they contain. Human Matrilin-3 shares 82% aa sequence identity with mouse Matrilin-3. Within the first VWA domain, human Matrilin-3 shares approximately 55% aa sequence identity with human Matrilin-1, -2, and -4. The coiled coil domain of Matrilin-3 mediates disulfide-linked homo-oligomerization, with tetramer formation being the most dominant (5 - 7). It can also assemble into hetero-oligomers with Matrilin-1 (5 - 7). Matrilin-3 is more plentiful than Matrilin-1 in the proliferative zone of the growth plate, whereas the reverse is true in the maturation zone (5). Matrilin-3 interacts directly with Collagen IX and COMP (8, 9). In the absence of Collagen IX, the expression of Matrilin-3 is unchanged, although it is retained inside chondrocytes and is not incorporated into the matrix (9). Matrilin-3 also associates with smaller cartilage fibrils independent of Collagen IX (9). Matrilin-3 knockout mice do not display any obvious abnormalities, suggesting that other molecules may compensate for the lack of Matrilin-3 (10). In contrast, intracellular retention of Matrilin-3 with particular point mutations in the VWA domain results in multiple epiphyseal dysplasia (11 - 13). A point mutation in the first EGF-like repeat which has been linked to hand osteoarthritis does not prevent Matrilin-3 secretion (13).

Item	Value
References	Wagener, R. et al. (2005) FEBS Lett. 579:3323. Deak, F. et al. (1999) Matrix Biol. 18:55. Whittaker, C.A. and R.O. Hynes (2002) Mol. Biol. Cell 13:3369. Belluoccio, D. et al. (1998) Genomics 53:391. Zhang, Y. and Q. Chen (2000) J. Biol. Chem. 275:32628. Klatt, A.R. et al. (2000) J. Biol. Chem. 275:3999. Frank, S. et al. (2002) J. Biol. Chem. 277:19071. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294. Budde, B. et al. (2005) Mol. Cell. Biol. 25:10465. Ko, Y. et al. (2004) Mol. Cell. Biol. 24:1691. Jackson, G.C. et al. (2004) J. Med. Genet. 41:52. Cotterill, S.L. et al. (2005) Hum. Mutat. 26:557. Otten, C. et al. (2005) J. Med. Genet. 42:774.
Entrez Gene IDs	4148 (Human); 17182 (Mouse)
Alternate Names	DIPOA, EDM5, HOA, Matrilin-3, Matrilin3, NDUFC2, OADIP, OS2, matrilin 3

Citations for Recombinant Human Matrilin-3 Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Matrilin-3 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

XD Lu et al. (2020-05-01), Matrilin-3 alleviates extracellular matrix degradation of nucleus pulposus cells via induction of IL-1 receptor antagonist Eur Rev Med Pharmacol Sci, 2020-024(10):5231-5241.
PMID: 32495856
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Yang X et al. (2014-10-20), Matrilin-3 inhibits chondrocyte hypertrophy as a bone morphogenetic protein-2 antagonist. J Biol Chem, 2014-1289(50):34768-79.
PMID: 25331953
Species: Human
Sample Types: Tissue Homogenates
Applications: Bioassay

Wang Q et al. (2011-11-06), Identification of a central role for complement in osteoarthritis. Nat. Med., 2011-117(12):1674-9.
PMID: 22057346
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay Substrate

Hills R et al. (2007-02-20), Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate. J. Biol. Chem., 2007-0282(15):11101-9.
PMID: 17311924

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Recombinant Human Matrilin-3 Protein, CF

Catalog # 3017-MN | R&D Systems, Inc. a Bio-Techne Brand

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