Recombinant Human JAK1 kinase domain His-tag Protein, CF

Name: Recombinant Human JAK1 kinase domain His-tag Protein, CF
Brand: R&D Systems
SKU: 11738-J1

R&D Systems, part of Bio-Techne | Catalog # 11738-J1

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Product Datasheet / COA / SDS

Key Product Details

Source:	HEK293
Applications:	Bioactivity

View Full Technical Details for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human Jak1 protein

Met

6-His tag

Sumo-tag
(mutated, uncleavable)

3c Protease site

Human JAK1
(Ala561-Lys1154)
Accession # P23458.2

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Protein identity confirmed by mass spectrometry.

Predicted Molecular Mass

81 kDa

SDS-PAGE

81 kDa

Activity

Measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a peptide substrate.

The specific activity is >110 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human JAK1 kinase domain His-tag Protein, CF

	Recombinant Human JAK1 kinase domain His-tag Enzyme Activity. Recombinant Human JAK1 kinase domain His-tag (Catalog # 11738-J1) is measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a peptide substrate.
	Recombinant Human JAK1 kinase domain His-tag SDS-PAGE. 2 μg/lane of Recombinant Human JAK1 kinase domain His-tag (Catalog # 11738-J1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-89 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11738-J1

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Jak1

Janus Kinase 1 (JAK1) is one of four members of the JAK, or "just another kinase", family of intracellular, non-receptor tyrosine kinases (1) that noncovalently associate with Type I and Type II cytokine receptor cytoplasmic regions to mediate receptor tyrosine and subsequent STAT protein phosphorylation. JAK1 is broadly expressed in many cell types and contains several conserved JAK homology (JH) domains that compose an N-terminal FERM domain followed by a Src homology (SH) domain that associate and mediate interaction with the cytokine receptors (2,3) and a pseudokinase domain with no catalytic activity that regulates the activity of the catalytic kinase domain present at the C-terminus (4). JAK/STAT signaling is activated by over 50 cytokines that bind to the major cytokine receptors prompting auto-activation of JAKs by transphosphorylation and subsequent phosphorylation of the receptors to promote STAT binding (1,4). Once STAT proteins are bound, JAKs phosphorylate STAT causing dissociation, dimerization, and translocation to the nucleus where they function as transcription factors and play a role in the control of hematopoiesis, the immune response, embryogenesis, inflammation, and cancer (5-8). In addition, JAK1 activating mutations have been directly reported in Castleman's disease (9) and T-cell malignancies such as activating T-ALL, Sezary Syndrome, and T-cell prolymphocytic leukemia (10-12). There is interest in development of JAK inhibitors as a therapeutic target to prevent phosphorylation of their substrates and downstream signaling pathways. Prior clinical studies targeting JAK1 have been focused on rheumatoid arthritis, myelofibrosis, and dermatology-related conditions such as psoriasis (1,13-15).

Item	Value
References	Agashe, R.P. et al. (2022) Mol. Cancer Ther. 21:1757. Leonard, W.J. and J.J. O'Shea (1998) Annu. Rev. Immunol. 16:293. Wallweber, H.J. et al. (2014) Nat. Struct. Mol. Biol. 21:443. Garrido-Trigo, A. et al. (2020) J. Crohns Colitis. 14:S713. Morris, R. et al. (2018) Protein. Sci. 27:1984. Loh, C.Y. et al. (2019) Front. Oncol. 9:48. Fragoulis, G.E. et al. (2019) Rheumatology 58:i43. Brooks, A.J. and T. Putoczki. (2020) Cancers 12:1971. Patel, M. et al. (2017) JAMA Dermatol. 153:449. Kiel, M.J. et. al. (2015) Nat. Commun. 6:8470. Govaerts, I. et al. (2019) Hemasphere 3:e313. Wahnschaffe, L. et. al. (2019) Cancers 11:1833. Harrington, R. et al. (2020) J. Inflamm. Res. 13:519. Plosker, G.L. (2015) Drugs 75:297. Papp, K. et al. (2021) J. Am. Acad. Dermatol. 85:863.
Long Name	Janus Kinase 1
Entrez Gene IDs	3716 (Human)
Alternate Names	EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, JTK3, Jak1, Janus kinase 1EC 2.7.10.2

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Recombinant Human JAK1 kinase domain His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11738-J1

Key Product Details

Product Specifications for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Scientific Data Examples for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Jak1

Customer Reviews for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Product Documents for Recombinant Human JAK1 kinase domain His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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