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Recombinant Human ITIH3 His-tag Protein, CF

Catalog # 10427-IT | R&D Systems, Inc. a Bio-Techne Brand
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10427-IT-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ITIH3 protein
Ser34-Asp651, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser34

Predicted Molecular Mass

70 kDa

SDS-PAGE

81-98 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ITIH3 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 1-10 μg/mL

Scientific Data Images for Recombinant Human ITIH3 His-tag Protein, CF

Recombinant Human ITIH3 His-tag Protein SDS-PAGE

Recombinant Human ITIH3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ITIH3 His-tag Protein (Catalog # 10427-IT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at approximately 86 and 93 kDa under reducing conditions.

Formulation, Preparation and Storage

10427-IT
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH3

Inter-alpha-trypsin inhibitor heavy chain 3 (ITIH3) is a heavy chain (HC) member of the ITIH family that is synthesized in the liver and circulates in the plasma (1). ITIH3 contains a signal sequence, propeptide, and a conserved von-Willebrand type A domain similar to other HCs in the family. The ITIH3 c-termini is trimmed to reveal a c-terminal aspartic acid residue that enables crosslinking of the HC to chondroitin sulfate (CS) (1,2). ITIH3 represents the single HC3 component of pre-a-trypsin inhibitor (PaI). PaI is a protease inhibitor involved in extracellular matrix stabilization that is composed of a single subunit of HC3 and bikunin, which are linked together via a CS moiety (1,3).  ITIH3 can alternatively be associated with hyaluronan (HA) to form the Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in the context of inflammatory processes and ovulation (3,4). ITIH3 is up-regulated in acute inflammation via interleukins and PaI is considered a positive acute-phase protein (1,5). ITIH3 is implicated as a diagnostic and prognostic marker in several diseases including colorectal cancer (6), gastric cancer (7), pancreatic cancer (8), and Sjogrens's syndrome with rheumatoid arthritis (9). ITIH3 has been shown to increase cell attachment and reduce the number of metastases in an antitumoral role (10). Recombinant Human ITIH3 activity is measured though binding to Tumor necrosis factor-stimulated gene-6 (TSG-6). TSG-6 has high affinity for HC and plays an important role in the transfer of HC to HA matrix by forming a TSG-6:HC complex intermediate required for the transfer (4,11).

References

  1. Salier, J.P. et al.(1996) Biochem. J. 315:1.
  2. Bourguignon, J. et al. (1993) Eur. J. Biochem. 212:771.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Rugg. M.S. et al. (2005) J. Biol. Chem. 280:25674.
  5. Daveau, M. et al. (1993) Biochem. J. 292:485.
  6. Jiang, X. et al. (2019) Dis. Markers. 2019:5069614.
  7. Chong, P.K. et al. (2010) J. Proteome Res. 9:3671.
  8. Liu, X. et al. (2017) Br. J. Cancer 117:1846.
  9. Liao, C.C. et al. (2016) J. Proteomics. 141:1.
  10. Paris, S. et al. (2002) Int. J. Cancer 97:615.
  11. Baranova, N.S et al. (2013) J. Biol. Chem. 288:29642.

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 3

Alternate Names

H3P, ITI-HC3

Entrez Gene IDs

3699 (Human); 16426 (Mouse)

Gene Symbol

ITIH3

UniProt

Product Documents for Recombinant Human ITIH3 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ITIH3 His-tag Protein, CF

For research use only

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