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Recombinant Human ITIH1 His-tag Protein, CF

Catalog # 10268-IT | R&D Systems, Inc. a Bio-Techne Brand
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10268-IT-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ITIH1 protein
Ser30-Asp672, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser30

Predicted Molecular Mass

73 kDa

SDS-PAGE

79-82 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ITIH1 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 1-6 μg/mL

Scientific Data Images for Recombinant Human ITIH1 His-tag Protein, CF

Recombinant Human ITIH1 His-tag Protein Binding Activity

Recombinant Human ITIH1 His-tag Protein Binding Activity

When Recombinant Human ITIH1 (Catalog # 10268-IT) is coated at 2 µg/mL, 100 µL/well, Recombinant Human TSG-6 (Catalog # 2104-TS) binds with an ED50 of 1-6 µg/mL.
Recombinant Human ITIH1 His-tag Protein SDS-PAGE

Recombinant Human ITIH1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ITIH1 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 80 kDa under reducing conditions.

Formulation, Preparation and Storage

10268-IT
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH1

Inter-alpha-trypsin inhibitor heavy chain 1 (ITIH1) is a heavy chain (HC) member of the ITIH family that is synthesized in the liver and circulates in the plasma (1). ITIH1 contains a signal sequence, propeptide, and a conserved von-Willebrand type A domain as in other HCs in the family. ITIH1 also includes a c-terminal extension multicopper oxidase domain, present in ITIH3 as well, that is trimmed to reveal a c-terminal aspartic acid residue that enables crosslinking of the HC to chondroitin sulfate (CS) (1,2). ITIH1 represents the HC1 component of Inter-alpha -inhibitor.  Inter-alpha -inhibitor is a protease inhibitor composed of three subunits (HC1, HC2, and bikunin), which are linked together via a CS moiety (1,3). ITIH1 can alternatively be associated with hyaluronan (HA) to form the Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in the context of inflammatory processes and ovulation (3,4).  Tumor necrosis factor-stimulated gene-6 (TSG-6) has high affinity for heavy chains and plays an important role in the transfer of HC to HA matrix by forming a TSG-6:HC complex intermediate required for the transfer (4,5). Inter-alpha -inhibitor heavy chains potentiate CD-44-mediated leukocytes adhesion to hyaluronan substratum and recruit immune cells to sites of inflammation (6).  The SHAP-HA complex was found to be upregulated in patients at various clinical stages of chronic hepatitis (CH), liver cirrhosis (LC), and hepatocellular carcinoma (HCC) caused by infection with the hepatitis C or hepatitis B virus (7).  ITIH1 has been shown to increase cell attachment and reduce the number of metastases in an antitumoral role (8).

References

  1. Salier, J.P. et al. (1996) Biochem. J. 315:1.
  2. Jean, L. et al. (1997) Genomics 41:139.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  5. Baranova, N.S et al. (2013) J. Biol. Chem. 288: 29642.
  6. Zhuo, L. et al. (2006) J. Biol. Chem. 281:20303.
  7. Shen, L. et al.  (2006) Hepatol. Res. 34:178.
  8. Paris, S. et al. (2002) Int. J. Cancer 97:615.

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 1

Alternate Names

H1P, IATIH, IGHEP1, ITI-HC1, ITIH

Entrez Gene IDs

3697 (Human); 16424 (Mouse)

Gene Symbol

ITIH1

UniProt

Product Documents for Recombinant Human ITIH1 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ITIH1 His-tag Protein, CF

For research use only

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