What is the amino acid sequence of the acidic and basic tails?

Name: Recombinant Human Integrin alpha 5 beta 1 Protein, CF
Brand: R&D Systems
SKU: 3230-A5
Rating: 4 (3 reviews)

Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Catalog # 3230-A5 | R&D Systems, Inc. a Bio-Techne Brand

(3)

Citations (19)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P08648 (Integrin alpha 5) & P05556 (Integrin beta 1)
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Product Specifications for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Source

Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein

Human Integrin alpha 5 (Phe42-Tyr995) Accession # P08648	acidic tail
Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	basic tail
N-terminus	C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Phe42 (Integrin alpha 5) & No results obtained: Gln21 predicted (Integrin beta 1)

Predicted Molecular Mass

108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit)

SDS-PAGE

105-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 binds Bovine Fibronectin (Catalog # 1030-FN) coated at 2 µg/mL with an apparent K_d <0.5 nM in the presence of 1 mM MnCl₂.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3230-A5

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 5 beta 1

Integrin alpha 5/ beta 1, also known as VLA-5, is a widely expressed non-covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). The human Integrin alpha 5/CD49e cDNA encodes a 1049 amino acid (aa) precursor that includes a 41 aa signal sequence, a 954 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain. The ECD contains seven FG-GAP repeats and nine internal disulfide bonds (3). Within the ECD, human alpha 5 shares 90% and 46% aa sequence identity with mouse alpha 5 and human alpha 8, respectively, and less than 30% aa sequence identitity with other human alpha chains. The human Integrin beta 1/CD29 cDNA encodes a 798 aa precursor that includes a 20 aa signal sequence, a 708 aa ECD, a 23 aa transmembrane segment, and a 47 aa cytoplasmic domain. The ECD contains one vWF-A domain, four Cys-rich repeats, and 29 internal disulfide bonds (3). Five alternate splice forms of the cytoplasmic domain vary by 12 to 48 aa. Within the ECD, human beta 1 shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. It shares 35-45% aa sequence identity with other beta chains. alpha 5/ beta 1 binds fibronectin in both RGD-dependent and -independent manners (4, 5). alpha 5/ beta 1 is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGFR3 and Tie2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including HER2, uPAR, Galectin‑1, CTGF, and thrombin-cleaved Osteopontin (5, 10-13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14, 15).

Item	Value
References	Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183. Takagi, J. et al. (2003) EMBO J. 22:4607. Wei, Y. et al. (2005) J. Cell Biol. 168:501. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193. Kim, S. et al. (2000) Am. J. Pathol. 156:1345. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205. Cascone, I. et al. (2005) J. Cell Biol. 170:993. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266. Gao, R. and D.R. Brigstock (2005) Gut 129:1019 Yokosaki, Y. et al. (2005) Matrix Biol. 24:418. Carstanjen, D. et al. (2005) Transfusion 45:1192. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
Entrez Gene IDs	3678 (Human)
Alternate Names	CD49 antigen-like family member E, CD49e, FNRA, Fibronectin receptor subunit alpha, ITGA5/SLC9B1 fusion, Integrin alpha 5 beta 1, Integrin alpha-F, VLA-5, VLA5A, fibronectin receptor, alpha polypeptide, fibronectin receptor, alpha subunit, integrin alpha-5, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), very late activation protein 5, alpha subunit

Citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF (19)

Showing 1 - 10 of 19 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

K Ning et al. (2023-01-11), Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors Science Advances, 2023-09(2):eade0869.
PMID: 36630517
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation

N Gerwin et al. (2022-12-01), Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 2022-10(0).
PMID: 36456835
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Capture

C Cegarra et al. (2022-09-15), An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-017(9):e0274667.
PMID: 36108060
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

EG Norris et al. (2022-04-11), Receptor binding domain of SARS-CoV-2 is a functional alphav-integrin agonist bioRxiv : the preprint server for biology, 2022-00(0).
PMID: 35441172
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation

N Gerwin et al. (2022), Angiopoietin-like 3-derivative LNA043 for cartilage regeneration in osteoarthritis: a randomized phase 1 trial Nature Medicine, 0(0).
PMID: 36456835
Species: Human
Sample Types: Protein
Applications: Bioassay

F Irie et al. (2021-02-26), The Cell Surface Hyaluronidase TMEM2 Regulates Cell Adhesion and Migration via Degradation of Hyaluronan at Focal Adhesion Sites The Journal of Biological Chemistry, 2021-00(0):100481.
PMID: 33647313
Species: Human

Applications: ELISA Standard

S Rattila et al. (2019-10-31), Interaction of Pregnancy-Specific Glycoprotein 1 With Integrin alpha5beta1 Is a Modulator of Extravillous Trophoblast Functions Cells, 2019-18(11).
PMID: 31683744
Species: Human
Sample Types: Peptide
Applications: Bioassay

PR Kuninty et al. (2019-09-04), ITGA5 inhibition in pancreatic stellate cells attenuates desmoplasia and potentiates efficacy of chemotherapy in pancreatic cancer Sci Adv, 2019-05(9):eaax2770.
PMID: 31517053
Sample Types: Protein
Applications: Bioassay

EC Skoog et al. (2018-05-15), CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding MBio, 2018-09(3).
PMID: 29764950
Species: Human
Sample Types: Peptide
Applications: Bioassay

MA Barbhuiya et al. (2017-09-21), Therapeutic potential of an anti-angiogenic multimodal biomimetic peptide in hepatocellular carcinoma Oncotarget, 2017-08(60):101520-101534.
PMID: 29254183
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Developmet

(2016-12-06), Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains Sci Rep, 2016-16(0):38101.
PMID: 27922023
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Brown A et al. (2015-08-28), Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat. J Biol Chem, 2015-0290(42):25534-47.
PMID: 26318455
Species: Zebrafish
Sample Types: Embryo
Applications: In Vivo

Qiao L et al. (2014-03-26), Snail modulates the assembly of fibronectin via alpha5 integrin for myocardial migration in zebrafish embryos. Sci Rep, 2014-04(0):4470.
PMID: 24667151
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Stampolidis P et al. (2013-12-23), LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene, 2013-134(1):39-52.
PMID: 24362527
Sample Types: Recombinant Protein
Applications: Binding Assay

Kwon I et al. (2013-11-18), Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013-18(11):e81165.
PMID: 24260554
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Haidari M et al. (2012-07-25), Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells. J Biol Chem, 2012-0287(39):32981-92.
PMID: 22833667
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shroff K et al. (2012-02-29), PEGylated liposomal doxorubicin targeted to alpha5beta1-expressing MDA-MB-231 breast cancer cells. Langmuir, 2012-028(10):4729-36.
PMID: 22268611
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Martchenko M et al. (2010-08-16), Heterodimeric integrin complexes containing beta1-integrin promote internalization and lethality of anthrax toxin. Proc. Natl. Acad. Sci. U.S.A., 2010-0107(35):15583-8.
PMID: 20713715
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Jullienne B et al. (2009-12-01), Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid. Gene Ther., 2009-116(12):1405-15.
PMID: 19641530

There are no citations that match your criteria.

Showing 1 - 10 of 19 citations Showing All

Customer Reviews for Recombinant Human Integrin alpha 5 beta 1 Protein, CF (3)

4.3 out of 5

3 customer ratings

5 stars

33%

4 stars

67%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 3 of 3 reviews Showing All

Filter By:

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Name: Andres Ocampo

Application: In vitro bioactivity in cell culture

Reason for Rating: The product was easy to dissolve and induced an expected response in cell culture.

Verified Customer | Posted 10/11/2022

Integrin was used to stimulate a pro-inflammatory activity on rats’ synoviocytes. For this, primary synoviocytes were isolated from rats’ articulations and treated with the integrin. Results showed a pro-inflammatory production of IL-1ra, IL-6, and TNF-a.
Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Name: Amruta Narayanappa

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 11/27/2020
Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 04/30/2019

There are no reviews that match your criteria.

Showing 1 - 3 of 3 reviews Showing All

FAQs for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

What is the amino acid sequence of the acidic and basic tails?

Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Catalog # 3230-A5 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Integrin alpha 5 beta 1

Citations for Recombinant Human Integrin alpha 5 beta 1 Protein, CF (19)

Customer Reviews for Recombinant Human Integrin alpha 5 beta 1 Protein, CF (3)

Customer Images

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Recombinant Human Integrin alpha 5 beta 1 Protein, CF

FAQs for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Product Documents for Recombinant Human Integrin alpha 5 beta 1 Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne 品牌

保持关注