Recombinant Human IL-5 Protein Best Seller

Name: Recombinant Human IL-5 Protein
Brand: R&D Systems
SKU: 205-IL
Price: 205 USD

Catalog # 205-IL | R&D Systems, Inc. a Bio-Techne Brand

Citations (37)

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Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (stably transfected)
Accession #:	P05113
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-5 Protein

Product Specifications for Recombinant Human IL-5 Protein

Source	Spodoptera frugiperda, Sf 21 (stably transfected)-derived human IL-5 protein Ile20-Ser134
Purity	>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ile20
Predicted Molecular Mass	13 kDa (monomer)
SDS-PAGE	13-15 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.04-0.2 ng/mL.

Scientific Data Examples for Recombinant Human IL-5 Protein

	Recombinant Human IL-5 Protein Bioactivity Recombinant Human IL-5 (Catalog # 205-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is 0.04-0.2 ng/mL.
	Recombinant Human IL-5 Protein SDS-PAGE 1 μg/lane of Recombinant Human IL-5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13-15 kDa and 28-32 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

205-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

205-IL/CF

Carrier Free

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross‑reactivity with mouse IL-5. IL-5 is primarily produced by CD4⁺ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34⁺ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

Item	Value
References	Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Minamitake, Y. et al. (1990) J. Biochem. 107:292. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317. Sakuishi, K. et al. (2007) J. Immunol. 179:3452. Clutterbuck, E. J. et al. (1989) Blood 73:1504. Cameron, L. et al. (2000) J. Immunol. 164:1538. Tavernier, J. et al. (1991) Cell 66:1175. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342. Tavernier, J. et al. (2000) Blood 95:1600. Kopf, M. et al. (1996) Immunity 4:15. Horikawa, K. and K. Takatsu (2006) Immunology 118:497. Denburg, J. A. et al. (1991) Blood 77:1462.
Long Name	Interleukin 5
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names	B-cell differentiation factor I, BCDF mu, BCGFII, EDF, Eo-CSF, Eosinophil differentiation factor, IL-5, IL-5T-cell replacing factor, IL5, TRF, TRFB cell differentiation factor I, interleukin 5 (colony-stimulating factor, eosinophil), interleukin-5

Citations for Recombinant Human IL-5 Protein (37)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-5 Protein include:

Species: Nematode - Brugia malayi
Sample Types: Complex Sample Type
Applications: ELISA Detection

R Mejia et al. (2022-04-25), Localization and RNA Interference-Driven Inhibition of a Brugia malayi-Encoded Interleukin-5 Receptor Binding Protein Infection and Immunity, 2022-00(0):e0031721.
PMID: 35467360
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Imoto et al. (2020-11-18), Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis Biomolecules, 2020-110(11).
PMID: 33218117
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Yan et al. (2020-06-30), T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-031(13):107820.
PMID: 32610123
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Y Cao et al. (2020-04-10), Single-site, five-year experience with human eosinophil isolation by density gradient centrifugation and CD16 immunomagnetic negative separation BMC Res Notes, 2020-013(1):211.
PMID: 32276656
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ME Coden et al. (2019-12-09), Fibrinogen Is a Specific Trigger for Cytolytic Eosinophil Degranulation J. Immunol., 2019-10(0).
PMID: 31818982
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Qin et al. (2019-03-01), The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019-0117(0):84-90.
PMID: 30831444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Karina T Barretto et al. (2018-07-26), Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin. PLoS ONE, 2018-00(0):1932-6203.
PMID: 30048528
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Bortnov et al. (2018-06-28), Myeloid-derived growth factor is a resident endoplasmic reticulum protein J. Biol. Chem., 2018-00(0).
PMID: 29954947
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Bucher et al. (2018-06-18), Interleukin-5 suppresses Vascular Endothelial Growth Factor-induced angiogenesis through STAT5 signaling Cytokine, 2018-00(0).
PMID: 29921484
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

F Zellweger et al. (2018-05-01), IL-3 but not monomeric IgE regulates Fc?RI levels and cell survival in primary human basophils Cell Death Dis, 2018-09(5):510.
PMID: 29724998
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JT Schroeder et al. (2017-06-26), Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen J. Immunol., 2017-00(0).
PMID: 28652400
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MW Johansson et al. (2017-05-05), IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration Clin. Exp. Allergy, 2017-00(0).
PMID: 28378503
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Toyama et al. (2017-02-16), Human eosinophils constitutively express a unique serine protease, PRSS33 Allergol Int, 2017-00(0).
PMID: 28216055
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-09-30), Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes Immunology, 2016-00(0).
PMID: 27568595
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-08-20), The effect of hepatocyte growth factor on secretory functions in human eosinophils Cytokine, 2016-088(0):45-50.
PMID: 27552115
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-05-09), Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients J Immunol, 2016-00(0).
PMID: 27183585
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Schwartz C et al. (2015-04-10), Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis. Blood, 2015-0125(25):3896-904.
PMID: 25862560
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Konno et al. (2015-03-19), Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis. PLoS ONE, 2015-010(3):e0120386.
PMID: 25790291
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yao et al. (2015-03-18), IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model. Respir Res, 2015-016(0):39.
PMID: 25889697
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Dyer et al. (2015-03-16), Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24). PLoS ONE, 2015-010(3):e0118887.
PMID: 25774667
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zafra M et al. (2015-03-10), SOCS3 silencing attenuates eosinophil functions in asthma patients. Int J Mol Sci, 2015-016(3):5434-51.
PMID: 25764157
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Burnham M et al. (2014-08-14), Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils. PLoS ONE, 2014-09(8):e103122.
PMID: 25121926
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Awad A et al. (2014-04-11), Natural killer cells induce eosinophil activation and apoptosis. PLoS ONE, 2014-09(4):e94492.
PMID: 24727794
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kankaanranta H et al. (2014-02-28), Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. PLoS ONE, 2014-09(2):e90298.
PMID: 24587316
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Suzuki et al. (2014-01-07), Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes. J Biol Chem, 2014-0289(8):5330-9.
PMID: 24398680
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Oh et al. (2013-04-19), Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils. J Immunol, 2013-0190(10):4937-45.
PMID: 23606538
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Puxeddu I et al. (2009-10-05), Osteopontin is expressed and functional in human eosinophils. Allergy, 2009-165(2):168-74.
PMID: 19804447
Sample Types: N/A
Applications: ELISA (Standard)

Qui H et al. (2007-11-01), Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-153(11):2010-2.
PMID: 18030696
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Vassina EM et al. (2006-07-01), cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis. Eur. J. Immunol., 2006-036(7):1975-84.
PMID: 16761316
Sample Types: N/A
Applications: ELISA (Standard)

Gounni Abdelilah S et al. (2006-06-16), Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006-0120(2):220-31.
PMID: 16782408
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brooks AM et al. (2006-05-25), Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Am. J. Respir. Cell Mol. Biol., 2006-035(4):503-11.
PMID: 16728704
Species: Guinea Pig
Sample Types: Whole Cells
Applications: Bioassay

Liu F et al. (2005-05-01), Pharmacological characterization of guinea pig chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2). Prostaglandins Other Lipid Mediat., 2005-076(1):133-47.
PMID: 15967168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fujitsu Y et al. (2005-03-01), Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13. Invest. Ophthalmol. Vis. Sci., 2005-046(3):797-802.
PMID: 15728533
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kao CY et al. (2004-09-01), IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol., 2004-0173(5):3482-91.
PMID: 15322213
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johansson MW et al. (2004-06-25), Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes. Am. J. Respir. Cell Mol. Biol., 2004-031(4):413-22.
PMID: 15220135
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hellman C et al. (2003-01-01), Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-0131(1):75-81.
PMID: 12519389
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu X et al. (2002-05-01), Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2002-0282(5):L1049-56.
PMID: 11943670

Recombinant Human IL-5 Protein Best Seller

Catalog # 205-IL | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human IL-5 Protein

Scientific Data Examples for Recombinant Human IL-5 Protein

Formulation, Preparation and Storage

Carrier Free

205-IL

Carrier

205-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-5

Citations for Recombinant Human IL-5 Protein (37)

Product Documents for Recombinant Human IL-5 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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