Recombinant Human IFN-alpha A Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11101-1
Key Product Details
- R&D Systems E. coli-derived Recombinant Human IFN-alpha A Protein (11101-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived human IFN-alpha A protein
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
19.2 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as
described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"
J. Virol. 37, 755-758;
Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for
Interferon," in
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral
assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units
are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-
alphaA) provided by
the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in
Methods in
Enzymology
(S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells
with vesicular stomatitis virus (VSV).
Formulation, Preparation and Storage
11101-1
| Formulation | Phosphate buffered saline (PBS) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Background: IFN-alpha 2/IFNA2
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Long Name
Interferon alpha 2
Alternate Names
Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2
Gene Symbol
IFNA2
UniProt
Additional IFN-alpha 2/IFNA2 Products
Product Documents for Recombinant Human IFN-alpha A Protein, CF
Product Specific Notices for Recombinant Human IFN-alpha A Protein, CF
For research use only
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