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Recombinant Human ICOS Fc Chimera Protein, CF

Catalog # 169-CS | R&D Systems, Inc. a Bio-Techne Brand
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169-CS-050

Key Product Details

Accession #

Source

NS0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ICOS protein
Human ICOS
(Glu21-Phe141)
Accession # Q9Y6W8
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

40.3 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Images for Recombinant Human ICOS Fc Chimera Protein, CF

Recombinant Human ICOS Fc Chimera Protein Bioactivity

Recombinant Human ICOS Fc Chimera Protein Bioactivity

Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/Fc Chimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1-4 µg/mL.

Formulation, Preparation and Storage

169-CS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

Inducible Co-stimulator

Alternate Names

AILIM, CD278, CRP-1

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Gene Symbol

ICOS

UniProt

Product Documents for Recombinant Human ICOS Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ICOS Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human ICOS Fc Chimera Protein, CF (3)

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Customer Reviews for Recombinant Human ICOS Fc Chimera Protein, CF (6)

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  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Sarah Garrett
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/15/2019
    Protein A beads were precoated with hICOS-L protein and incubated with increasing concentration of hIgG1 control or ICOS hIgG1 to a final concentration of 0 to 500nM. Binding was monitored by flow cytometry using anti-human 488 secondary antibody for detection.
    Recombinant Human ICOS Fc Chimera Protein, CF 169-CS
  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Travis Durkin
    Application: Characterization prior to use
    Verified Customer | Posted 07/08/2019
    Recombinant Human ICOS Fc Chimera Protein, CF 169-CS
  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Media additive for protein or antibody production
    Verified Customer | Posted 03/25/2019
  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: SDS-PAGE Control
    Verified Customer | Posted 10/24/2018
  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/12/2018
  • Recombinant Human ICOS Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Apoptosis assay
    Verified Customer | Posted 07/11/2018

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