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Recombinant Human ICOS Fc Chimera Protein, CF
Catalog # 169-CS | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
NS0
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICOS protein
| Human ICOS (Glu21-Phe141) Accession # Q9Y6W8 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu21
Predicted Molecular Mass
40.3 kDa (monomer)
SDS-PAGE
50-60 kDa, reducing conditions
Activity
Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
Scientific Data Images for Recombinant Human ICOS Fc Chimera Protein, CF
Recombinant Human ICOS Fc Chimera Protein Bioactivity
Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/Fc Chimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1-4 µg/mL.Formulation, Preparation and Storage
169-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICOS
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Product Documents for Recombinant Human ICOS Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ICOS Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human ICOS Fc Chimera Protein, CF (3)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human ICOS Fc Chimera Protein, CF include:
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Species: N/A
Sample Types: N/A
Applications: ELISA StandardK McKinski et al. (2021-06-27), Orthogonal quantification of soluble inducible T-cell costimulator (ICOS) in healthy and diseased human serum Journal of pharmaceutical analysis, 2021-012(2):317-323.
PMID: 35582393 -
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPRKH Koh et al. (2019-03-18), Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist J. Clin. Invest., 2019-0129(4):1713-1726.
PMID: 30747722 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayMesturini R et al. (2006-10-01), ICOS cooperates with CD28, IL-2, and IFN-gamma and modulates activation of human naive CD4+ T cells. Eur. J. Immunol., 2006-136(10):2601-12.
PMID: 17013990
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Customer Reviews for Recombinant Human ICOS Fc Chimera Protein, CF (6)
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6 customer
ratings
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/15/2019Protein A beads were precoated with hICOS-L protein and incubated with increasing concentration of hIgG1 control or ICOS hIgG1 to a final concentration of 0 to 500nM. Binding was monitored by flow cytometry using anti-human 488 secondary antibody for detection.
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Application: Characterization prior to useVerified Customer | Posted 07/08/2019
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Application: Media additive for protein or antibody productionVerified Customer | Posted 03/25/2019
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Application: SDS-PAGE ControlVerified Customer | Posted 10/24/2018
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 10/12/2018
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Application: Apoptosis assayVerified Customer | Posted 07/11/2018
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