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Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

Catalog # 10573-HE | R&D Systems, Inc. a Bio-Techne Brand
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10573-HE-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Hepassocin/FGL1 protein
MD
Human IgG1
(Pro100-Lys330)
IEGR Human Hepassocin/FGF1
(Leu23-Ile312)
Accession # Q08830.3
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

61 kDa

SDS-PAGE

60-65 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.

Scientific Data Images for Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

Recombinant Human Hepassocin/FCL1 Fc Chimera Protein Binding Activity.

When Recombinant Human LAG-3 Fc Chimera (2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.

Recombinant Human Hepassocin/FGL1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of 2 μg/lane of Recombinant Human Hepassocin/FGL1 Fc Chimera Protein (Catalog # 10573-HE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-65 kDa and 120-130 kDa, respectively.

Formulation, Preparation and Storage

10573-HE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

References

  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

Product Documents for Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

For research use only

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