Recombinant Human LAG-3 Fc Chimera Protein, CF Best Seller

Name: Recombinant Human LAG-3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 2319-L3
Rating: 5 (4 reviews)

Catalog # 2319-L3 | R&D Systems, Inc. a Bio-Techne Brand

(4)

Citations (4)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P18627
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LAG-3 Fc Chimera Protein, CF

Product Specifications for Recombinant Human LAG-3 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human LAG-3 protein

Human LAG-3 (Leu23-Leu450) Accession # P18627	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu23

Predicted Molecular Mass

72.7 kDa (monomer)

SDS-PAGE

75-90 kDa, under reducing conditions.

Activity

Measured by its ability to induce TNF-alpha secretion by JAWSII mouse immature dendritic cells. The ED₅₀ for this effect is 1-4 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2319-L3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitute 30 minutes prior to use with minimal agitation. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3), designated CD223, is a 70 kDa type I transmembrane protein that is a member of the immunoglobulin superfamily (IgSF) (1, 2). LAG-3 shares approximately 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity, providing negative regulation of T cell receptor signaling (1, 2). Human LAG-3 cDNA encodes 525 amino acids (aa) that include a 28 aa signal sequence, a 422 aa extracellular domain (ECD) with four Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. Within the ECD, human LAG-3 shares 70%, 67%, 76%, and 73% aa sequence identity with mouse, rat, porcine, and bovine LAG-3, respectively. LAG-3 is expressed on activated CD4⁺ and CD8⁺ T cells, NK cells, and plasmacytoid dendritic cells (pDC), but not on resting T cells (1-3). LAG-3 on activated CD4⁺CD25⁺ T_reg cells plays a role in their suppressive activity (4). LAG-3 limits the expansion of activated T cells and pDC in response to selected stimuli (3-5). A soluble 54 kDa form, sLAG-3, can be shed by metalloproteinases ADAM10 and TACE/ADAM17 (6, 7). While monomeric sLAG-3 itself may be inactive, shedding allows for normal T cell activation by removing negative regulation (7). Binding of a homodimerized sLAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC, and secretion of cytokines such as IFN-gamma and TNF-alpha by type 1 cytotoxic CD8⁺ T cells and NK cells (8, 9). sLAG-3/Ig has been used as a potential adjuvant to stimulate a cytotoxic anti-cancer immune response (9, 10). In mice, deletion of LAG-3 and another negative regulator, PD-1, facilitates anti-cancer response but also blocks self-tolerance and increases susceptibility to autoimmune diseases (11, 12). In humans, antibody-mediated down‑regulation of LAG-3 and PD-1 allows more effective control of chronic malaria, while in NOD (non‑obese diabetic) mice, deletion of LAG-3 alone accelerates diabetes (12-14).

Item	Value
References	Triebel, F. et al. (1990) J. Exp. Med. 171:1393. Baixeras, E. et al. (1992) J. Exp. Med 176:327. Workman, C.J. et al. (2004) J. Immunol. 172:5450. Huang, C.T. et al. (2004) Immunity 21:503. Workman, C.J. et al. (2009) J. Immunol. 182:1885. Li, N. et al. (2004) J. Immunol. 173:6806. Li, N. et al. (2007) EMBO J. 26:494. Andreae, S. et al. (2003) Blood 102:2130. Brignone, C. et al. (2007) J. Immunol. 179:4202. Brignone, C. et al. (2010) J. Transl. Med. 8:71. Woo, S.R. et al. (2011) Cancer Res. 72:917. Okazaki, T. et al. (2011) J. Exp. Med. 208:395. Bettini, M. et al. (2011) J. Immunol. 187:3493. Butler, N.S. et al. (2012) Nat. Immunol. 13:188.
Long Name	Lymphocyte-activation Gene 3
Entrez Gene IDs	3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
Alternate Names	CD223, CD223 antigen, LAG-3, LAG3, Secreted lymphocyte activation gene 3 protein, lymphocyte activating 3, lymphocyte activation gene 3 protein, lymphocyte-activation gene 3, sLAG-3

Citations for Recombinant Human LAG-3 Fc Chimera Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LAG-3 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

P Fittje et al. (2022-06-24), HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells Oncogene, 2022-018(6):e1010572.
PMID: 35749424
Species: Human
Sample Types: Protein
Applications: Bioassay

CJ Edwards et al. (2021-12-30), The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity British Journal of Cancer, 2021-10(0).
PMID: 34969998
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Weinberg et al. (2017-06-12), Varicella-Zoster Virus-Specific Cellular Immune Responses to the Live Attenuated Zoster Vaccine in Young and Older Adults J. Immunol., 2017-00(0).
PMID: 28607114
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tian X et al. (2015-03-16), The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects. J Immunol, 2015-0194(8):3873-82.
PMID: 25780040

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Customer Reviews for Recombinant Human LAG-3 Fc Chimera Protein, CF (4)

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Recombinant Human LAG-3 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: CellProlif

Verified Customer | Posted 03/25/2019
Recombinant Human LAG-3 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: Apoptosis assay

Verified Customer | Posted 07/11/2018
Recombinant Human LAG-3 Fc Chimera Protein, CF

Name: Jenna Nguyen

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 11/03/2017
Recombinant Human LAG-3 Fc Chimera Protein, CF

Name: Anonymous

Application: FACS sorting

Verified Customer | Posted 06/16/2017

Yeast scFv that bind to LAG-3-Fc are in the upper right quadrant. The expected number of binders was observed and were later confirmed to be true LAG-3 binders.

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Product Documents for Recombinant Human LAG-3 Fc Chimera Protein, CF

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Recombinant Human LAG-3 Fc Chimera Protein, CF Best Seller

Catalog # 2319-L3 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human LAG-3 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LAG-3

Citations for Recombinant Human LAG-3 Fc Chimera Protein, CF (4)

Customer Reviews for Recombinant Human LAG-3 Fc Chimera Protein, CF (4)

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Recombinant Human LAG-3 Fc Chimera Protein, CF

Recombinant Human LAG-3 Fc Chimera Protein, CF

Recombinant Human LAG-3 Fc Chimera Protein, CF

Recombinant Human LAG-3 Fc Chimera Protein, CF

Product Documents for Recombinant Human LAG-3 Fc Chimera Protein, CF

Product Datasheets

COA

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SDS

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