Recombinant Human GSK-3 beta His-tag Protein, CF

Name: Recombinant Human GSK-3 beta His-tag Protein, CF
Brand: R&D Systems
SKU: 11739-KS

R&D Systems, part of Bio-Techne | Catalog # 11739-KS

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Product Datasheet / COA / SDS

Key Product Details

Source:	HEK293
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Human GSK-3 beta His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human GSK-3 beta His-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human GSK-3 beta protein

Met

6-His tag

Sumo-tag
(mutated, uncleavable)

3c Protease site

Human GSK-3 beta
(Ser2-Thr420)
Accession # P49841.2

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Protein identity confirmed by mass spectrometry

Predicted Molecular Mass

60 kDa

SDS-PAGE

60 kDa

Activity

Measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a peptide substrate.

The specific activity is >200 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human GSK-3 beta His-tag Protein, CF

	Recombinant Human GSK-3 beta His-tag Enzyme Activity. Recombinant Human GSK-3 beta His-tag (Catalog # 11739-KS) is measured by its ability to transfer phosphate from adenosine triphosphate (ATP) to a peptide substrate.
	Recombinant Human GSK-3 beta His-tag SDS-PAGE. 2 μg/lane of Recombinant Human GSK-3 beta His-tag (Catalog # 11739-KS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-64 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11739-KS

Formulation	Supplied as a 0.2 μm filtered solution in MES, NaCl, Glycerol and DTT.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: GSK-3 beta

Glycogen synthase kinase-3 beta (GSK3B) is a ubiquitously expressed, highly conserved serine/threonine kinase (1-3) with key roles in the transduction of regulatory and proliferative signals at the cell membrane. GSK3B is one of two mammalian isoforms of GSK that are constitutively active and have high homology with a kinase domain and an N-terminal phosphorylation site that auto-inhibits its activity by competing with a binding site for preferred pre-phosphorylated substrates (1, 2, 4). The isoforms differ in their sequence similarity at both the N- and C-termini. The GSK3 alpha isoform is primarily cytosolic and has a glycine-rich N-terminal region that excludes it from the nucleus, whereas GSK3B can be found in the nucleus. The different localization differentiates GSK3B biological activity supported by the fact that the GSK3B knockout is embryonically lethal while GSK3 alpha expression is unable to compensate (3). GSK3 has been confirmed to have a diverse and broad range of substrates within multiple cell signaling pathways to regulate glucose metabolism, insulin activity, and energy homeostasis and plays a role in a wide range of cellular processes, such as cell proliferation, differentiation, apoptosis, the cell cycle, the immune response, and organ development (3, 5-6). GSK3B is thus implicated in multiple diseases such as cancer (2, 7) neurodegenerative diseases such as Alzheimer's, bipolar disorder (BPD), psychiatric disorders, Parkinson's, aging (8, 9), inflammatory conditions, and metabolic disorders such as diabetes, cardiovascular disease, and atherosclerosis (2-3, 7-11). There is interest in development of GSK3 inhibitors as a therapeutic target to prevent their ability to phosphorylate their substrates and prevent downstream signaling pathways; clinical trials targeting GSK3 inhibition have been primarily focused on cancers and neurodegenerative and neurological diseases (2-3, 9).

Item	Value
References	Dajani, R. et al. (2001) Cell 105:721. Mancinelli, R. et al. (2017) Oxidative Med. Cell. Longev. 2017:4629495. Wang, L. et al. (2022) Med. Res. Rev. 42:946. Cross, D.A.E. et al. (1995) Nature 378:785. Grimes C.A. and R.S. Jope (2001) Prog. Neurobiol. 65:391. Doble, B.W. and J.R. Woodgett (2003) J. Cell Sci. 116:1175. Lin, J. et al. (2020) Biochem. Biophys. Acta 1867:118659. Gianferrara, T. et al. (2022) Curr. Med. Chem. 29:4631. Arciniegas Ruiz, S.M.A. and H. Eldar-Finkelman (2022) Front. Mol. Neurosci 14:792364. Sharma, A.K. et al. (2020) Drug Discov. Today 25:633. Liang, L. et al. (2024) FASEB J. 38:e23458.
Long Name	Glycogen Synthase Kinase 3 beta
Entrez Gene IDs	2932 (Human); 56637 (Mouse); 84027 (Rat)
Alternate Names	EC 2.7.11, EC 2.7.11.26, GSK-3 beta, GSK3 beta, GSK3B, GSK3beta isoform, glycogen synthase kinase 3 beta, glycogen synthase kinase-3 beta

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Recombinant Human GSK-3 beta His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11739-KS

Key Product Details

Product Specifications for Recombinant Human GSK-3 beta His-tag Protein, CF

Scientific Data Examples for Recombinant Human GSK-3 beta His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: GSK-3 beta

Customer Reviews for Recombinant Human GSK-3 beta His-tag Protein, CF

Product Documents for Recombinant Human GSK-3 beta His-tag Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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