Recombinant Human Fibulin 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9559-FB

R&D Systems, part of Bio-Techne
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9559-FB-050
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Fibulin 2 Protein (9559-FB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession #

P98095-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibulin 2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fibulin 2 protein
Ala28-Leu1184 (Thr854Ala), with an N-terminal HA tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (of HA tag)

Predicted Molecular Mass

125 kDa

SDS-PAGE

150-175 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9559-FB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibulin 2

Fibulin 2 is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. Fibulin-2 consists of 1184 amino acids (aa) with a predicted molecular weight of ~125 kDa. The overall structure is common to fibulins: 3 Anaphylatoxin (AT)-like domains, 11 Epidermal Growth Factor (EGF)-like domains, and a Fibulin-type carboxy-terminal (FC) domain (1-4). Fibulin 2 is considered a Class I fibulin because the 400-residue N-terminus is divided into the Na and Nb sections, with the Na section containing 150-residues and 12 cysteines while the remaining Nb section is cysteine-free (2, 3). The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer (5). Fibulin 2 is highly conserved across species, with the human protein sharing 82% amino acid identity compared to both mouse and rat. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined (6). Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin (1, 2, 4, 7). It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors (1, 3, 4). There is also evidence that suggests that Fibulin 2 may play an indirect role in the neurogenesis of adult neural stem cells via interaction with integrins and TGF-beta 1 (8).

References

  1. Alcendor et al. (2011) Am. J. Pathol. 179:1443.
  2. Timpl et al. (2003) Nat. Rev. Mol. Cell Biol. 4:479.
  3. Kanan et al. (2014) J. Biol. Chem. 289:13419.
  4. Argraves et al. (2003) EMBO reports 4:1127.
  5. Pan et al. (1993) J. Cell Biol. 123:1269.
  6. Fontanil et al. (2014) Oncotarget 5:1253.
  7. El-Hallous et al. (2007) J. Biol. Chem. 282:8935.
  8. Radice et al. (2015) Mol. Cell. Neurosci. 67:75.

Alternate Names

FBLN2, FIBL-2, Fibulin-2

Entrez Gene IDs

2199 (Human); 14115 (Mouse); 282583 (Rat)

Gene Symbol

FBLN2

UniProt

P98095-1

Additional Fibulin 2 Products

Product Documents for Recombinant Human Fibulin 2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Fibulin 2 Protein, CF

For research use only

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