Recombinant Human Complement Component C1s Protein, CF

Name: Recombinant Human Complement Component C1s Protein, CF
Brand: R&D Systems
SKU: 2060-SE
Rating: 5 (1 reviews)

Catalog # 2060-SE | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P09871
Structure / Form:	Disulfide-linked heterodimer
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human Complement Component C1s Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Complement Component C1s protein Met1-Asp688, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu16 (A chain) & Ile438 (B chain)
Predicted Molecular Mass	47 kDa (A chain) & 28 kDa (B chain)
SDS-PAGE	62 kDa and 32 kDa, reducing conditions
Activity	Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >20,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2060-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl₂ and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Complement Component C1s

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain. The full-length (amino acid residues 1-688) of human C1s was expressed (3-5). The purified protein corresponded to the processed active form, with A and B chains starting at residue Glu16 and Ile438, respectively.

Item	Value
References	Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233. Tosi, M. et al. (1987) Biochemistry 26:8516. Mackinnon, C.M. et al. (1987) Eur. J. Biochem. 169:547. Kusumoto, H. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7307.
Entrez Gene IDs	716 (Human)
Alternate Names	C1 esterase, C1S, Complement Component C1s, Complement component 1 subcomponent s, EC 3.4.21, EC 3.4.21.42, FLJ44757, basic proline-rich peptide IB-1, complement C1s subcomponent, complement component 1, s subcomponent

Citations for Recombinant Human Complement Component C1s Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Complement Component C1s Protein, CF include:

Species: Bacteria - Borrelia burgdorferi
Sample Types: Recombinant Protein
Applications: ELISA Capture

X Tang et al. (2022-11-22), A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon gamma Cell Reports, 2022-141(8):111673.
PMID: 36417869
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Zhou ZH et al. (2012-04-13), Complement C1 esterase inhibitor levels linked to infections and contaminated heparin-associated adverse events. PLoS ONE, 2012-07(4):e34978.
PMID: 22514695

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Recombinant Human Complement Component C1s Protein, CF

Name: Guodong Zhang

Application: Enzymatic activity in vitro

Verified Customer | Posted 05/10/2018

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Product Documents for Recombinant Human Complement Component C1s Protein, CF

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Recombinant Human Complement Component C1s Protein, CF

Catalog # 2060-SE | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human Complement Component C1s Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Complement Component C1s

Citations for Recombinant Human Complement Component C1s Protein, CF (2)

Customer Reviews for Recombinant Human Complement Component C1s Protein, CF (1)

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Recombinant Human Complement Component C1s Protein, CF

Product Documents for Recombinant Human Complement Component C1s Protein, CF

Product Datasheets

COA

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SDS

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