Recombinant Human CD96 Fc Chimera Protein, CF

Name: Recombinant Human CD96 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9360-CD
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9360-CD

Citations (2)

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Key Product Details

Source:	HEK293
Accession #:	P40200-1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD96 Fc Chimera Protein, CF

Product Specifications for Recombinant Human CD96 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human CD96 protein

Human CD96 (Lys25-Met519) Accession # P40200-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Lys25

Predicted Molecular Mass

81 kDa

SDS-PAGE

131 - 160 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.

Scientific Data Examples for Recombinant Human CD96 Fc Chimera Protein, CF

	Recombinant Human CD96 Fc Chimera Protein Bioactivity When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED₅₀ of 0.3-1.8 µg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9360-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD96

Human CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4⁺ and CD8⁺ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). Mature human CD96 is a 564 amino acid (aa), type I transmembrane glycoprotein. It contains a 498 aa extracellular region that includes three Ig-like domains. The two N-terminal domains are V-type, while the distal domain is a C-type structure. Within the ECD, human CD96 shares 53% and 52% aa sequence identity with mouse CD96 and rat CD96, respectively (7). The ECD is highly N- and O-glycosylated (1). Humans express a splice variant with a 16 aa deletion in the second V-type domain, called CD96v2 (2). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). Mouse CD96 also binds nectin-1 (7). On NK cells, co-stimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11).

Item	Value
References	Wang, P.L. et al. (1992) J. Immunol. 148:2600. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008. Xie, W. et al. (2010) Cytometry A 77:840. Eriksson, E. M. et al. (2012) PLOS One 7:e51696. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959. Protein Accession # BAE32358. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207. Fuchs, A. et al. (2004) J. Immunol. 172:3394. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
Entrez Gene IDs	10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)
Alternate Names	CD96, CD96 antigenMGC22596, CD96 molecule, DKFZp667E2122, T cell-activated increased late expression protein, T-cell surface protein tactile, TACTILE, TACTILEincreased late expression

Citations for Recombinant Human CD96 Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CD96 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Joung et al. (2022), CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 13(1):1606.
PMID: 35338135
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

P Fittje et al. (2022), HIV-1 Nef-mediated downregulation of CD155 results in viral restriction by KIR2DL5+ NK cells Oncogene, 18(6):e1010572.
PMID: 35749424

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