Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Name: Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT2530

R&D Systems, part of Bio-Techne | Catalog # BT2530

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Product Datasheet / COA / SDS

Key Product Details

Source:	NS0
Accession #:	AHH15542.1
Structure / Form:	Biotinylated via amines
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Source

Mouse myeloma cell line, NS0-derived human CD155/PVR protein
Gly27-Asn343, with a C-terminal 6-His tag

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly27

Predicted Molecular Mass

35 kDa

SDS-PAGE

35 kDa

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human CD155/PVR His-tag (Catalog # BT2530) binds Recombinant Human TIGIT (A103) Fc Chimera (Catalog # 7898-TGB) with an ED₅₀ of 4.00-60.0 ng/mL.

Scientific Data Examples for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

	Biotinylated Recombinant Human CD155/PVR His-tag Protein Binding Activity. Biotinylated Recombinant Human CD155/PVR His-tag Protein (Catalog # BT2530) binds Recombinant Human TIGIT (A103) Fc Chimera (7898-TGB) with an ED₅₀ of 4.00‑60.0 ng/mL.
	Biotinylated Recombinant Human CD155/PVR His-tag Protein SDS-PAGE. 2 μg/lane of Biotinylated Recombinant Human CD155/PVR His-tag Protein (Catalog # BT2530) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT2530

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70 kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3). Mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. Within the ECD, human CD155 shares 45% aa sequence identity with mouse and rat CD155, and 52% with human Nectin-2. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (4). Human CD155 can also be spliced to generate secreted isoforms (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 12). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 13). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8+ thymocytes prevents their premature exit from the thymus (14). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 15). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 15, 16).

Item	Value
References	Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197. Mendelsohn, C.L. et al. (1989) Cell 56:855. Sato, T. et al. (2004) Genes to Cells 9:791. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235. Koike, S. et al. (1990) EMBO J. 9:3217. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214. Lozano, E. et al. (2013) J. Immunol. 191:3673. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251. Chan, C.J. et al. (2010) J. Immunol. 184:902. Qui, Q. et al. (2010) J. Immunol. 184:1681. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
Long Name	Poliovirus Receptor
Entrez Gene IDs	5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names	CD155, CD155 antigen, HVED, NECL5, Necl-5, Nectin-like protein 5, PVR, PVS, PVSFLJ25946, Tage4, nectin-like 5, poliovirus receptor

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Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT2530

Key Product Details

Product Specifications for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Scientific Data Examples for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD155/PVR

Customer Reviews for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Product Documents for Recombinant Human CD155/PVR His-tag Biotinylated Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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