Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI9174

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI9174

Product Datasheet / COA / SDS

Biotinylated

Catalog #

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Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	NP_006496.4
Structure / Form:	Disulfide-linked homodimer, Biotinylated via Avi-tag
Applications:	Bioactivity

View Full Technical Details for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Product Specifications for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human CD155/PVR protein

Human CD155/PVR (Asp28-Asn343) Accession # NP_006496.4	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp28

Predicted Molecular Mass

63 kDa

SDS-PAGE

86-98 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED₅₀ of 10-60 ng/mL.

Scientific Data Examples for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

	Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein Binding Activity When Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) is immobilized at 1 µg/mL, Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED₅₀ of 10-60 ng/mL.
	Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein SDS-PAGE 2 μg/lane of Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-98 kDa and 172-200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9174

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3). Mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. Within the ECD, human CD155 shares 45% aa sequence identity with mouse and rat CD155, and 52% with human Nectin-2. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (4). Human CD155 can also be spliced to generate secreted isoforms (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 12). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 13). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8⁺ thymocytes prevents their premature exit from the thymus (14). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 15). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 15, 16).

Item	Value
References	Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197. Mendelsohn, C.L. et al. (1989) Cell 56:855. Sato, T. et al. (2004) Genes to Cells 9:791. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235. Koike, S. et al. (1990) EMBO J. 9:3217. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11. Reymond, N. et al. (2004) J. Exp. Med. 199:1331. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214. Lozano, E. et al. (2013) J. Immunol. 191:3673. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251. Chan, C.J. et al. (2010) J. Immunol. 184:902. Qui, Q. et al. (2010) J. Immunol. 184:1681. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
Long Name	Poliovirus Receptor
Entrez Gene IDs	5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names	CD155, CD155 antigen, HVED, NECL5, Necl-5, Nectin-like protein 5, PVR, PVS, PVSFLJ25946, Tage4, nectin-like 5, poliovirus receptor

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Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

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Product Specifications for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Scientific Data Examples for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: CD155/PVR

Citations for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Product Documents for Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Product Datasheets

COA

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