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Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

Catalog # 10159-AN | R&D Systems, Inc. a Bio-Techne Brand
Mucin Stalk Chimera
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10159-AN-050

Key Product Details

Source

CHO

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 8/Betatrophin protein
Human Angiopoietin-like Protein 8/Betatrophin
(Ala22-Ala198)
Accession # Q6UXH0
Human Fractalkine mucin-like stalk
(Phe103-Thr338)
Accession # P78423
6-His tag
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

45 kDa

SDS-PAGE

70-93 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Angiopoietin-like Protein 8/Betatrophin that produces 50% of the optimal binding response is 0.15‑1.2 μg/mL.

Scientific Data Images for Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

Recombinant Human ANGPTL8/Betatrophin His-tag Protein Binding Activity

Recombinant Human ANGPTL8/Betatrophin His-tag Protein Binding Activity

When Recombinant Human Angiopoietin-like Protein 3/ANGPTL3 (Catalog # 3829-AN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human ANGPTL8/Betatrophin (Catalog # 10159-AN) binds with an ED50 of 0.15-1.2 µg/mL.

Formulation, Preparation and Storage

10159-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Human Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with mouse Betatrophin. Betatrophin is expressed in the liver, white adipose, and brown adipose tissues. It is a recently identified hormone which has been associated with two functionally important processes in the development of type 2 diabetes and obesity, insulin resistance as well as being a crucial modulator in lipid metabolism through regulation of serum triglyceride levels (2, 3). Betatrophin can form a complex with the N-terminal of ANGPTL3, and this complex is necessary for inhibition of lipoprotein lipase (LPL) activity and triglyceride modulation (4). Elevated levels of circulating betatrophin in the blood may be associated with gestational diabetes mellitus (GDM) and polycystic ovary syndrome (PCOS) (5, 6). Increased levels of Betatrophin in plasma and adipose tissue may also be associated with hypertension, coronary artery disease (CAD), and peripheral artery disease (PAD) (7, 8). The role of Betatrophin in inducing pancreatic beta-cell proliferation is controversial, as it did not control beta-cell expansion in the mouse model (9, 10). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders such as non-alcoholic fatty liver disease and renal dysfunction (11).

References

  1. Quagliarini, F. et al. (2012) PNAS 109:19751.
  2. Abu-Farha M. et al. (2017) Diabetes Metab Res Rev. 33:e2919.
  3. Zhang R. et al. (2012) Biochem Biophys Res Commun. 424:786
  4. Chi, X. et al. (2017) Molecular Metabolism 6:1137.
  5. Kong F.J. et al. (2017) PLoS One. 12:e0169941.
  6. Varikasuvu S.R. et al. (2019) Gynecol Endocrinol. 35:190
  7. Abu-Farha M. et al. (2018) Lipids Health Dis. 17:35
  8. Niki H. et al. (2019) J Atheroscler Thromb. 26:573.
  9. Abu-Farha M. et al. (2016) J Diabetes Res. 2016:4860595.
  10. Cox A.R. et al. PLoS One (2016) 11:e0159276.
  11. Luo, M. et al. (2018) Front Endocrino 9:169.

Alternate Names

Angiopoietin like Protein 8, ANGPTL8, Betatrophin, C19orf80, Gm6484, Lipasin, PRO1185, PVPA599, RIFL, TD26

Entrez Gene IDs

55908 (Human); 624219 (Mouse); 100361444 (Rat)

Gene Symbol

ANGPTL8

Product Documents for Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ANGPTL8/Betatrophin His-tag Protein, CF

For research use only

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