Recombinant Human Activin E Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11779-AE

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P58166.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all INHBE Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human INHBE protein
Thr237-Ser350

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met-Thr237

Predicted Molecular Mass

13 kDa

SDS-PAGE

12-15 kDa, under reducing conditions.

Activity

Measured by its ability to activate CAGA-SEAP reporter in HEK293 human embryonic kidney cells transfected with human ALK7. The ED50 for this effect is 1.00-10.0 ng/mL.

Scientific Data Images for Recombinant Human Activin E Protein, CF

Recombinant Human Activin E Protein Bioactivity.

Measured by its ability to activate ALK7-dependent SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 1.00-10.0 ng/mL.

Recombinant Human Activin E Protein SDS-PAGE.

2 μg/lane of Recombinant Human Activin E Protein (Catalog # 11779-AE) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 12-15 kDa.

Formulation, Preparation and Storage

11779-AE
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: INHBE

Activin E, encoded by the INHBE gene, is a liver-enriched member of the transforming growth factor-beta (TGF-beta) superfamily and functions as a hepatokine involved in systemic energy homeostasis. Structurally, it is a disulfide-linked homodimer of mature inhibin betaE subunits, sharing the conserved cystine-knot motif characteristic of TGF-beta ligands, which facilitates receptor binding and downstream signaling activation (1). Activin E signals through the type I receptor ALK7 (ACVR1C), activating the SMAD2/3 pathway and repressing PPARG target genes in adipose tissue (2). This signaling cascade suppresses beta-adrenergic-induced lipolysis, thereby promoting lipid retention in white adipose tissue and preventing excessive hepatic lipid influx during metabolic stress such as fasting (2, 3). In murine models, mRNA knocked down results in increased fat oxidation, reduced adiposity, and improved metabolic profiles under high-fat diet conditions (3). Conversely, hepatic overexpression of INHBE leads to visceral fat accumulation and adipocyte hypertrophy, indicating a maladaptive role in obesity (4). Rare loss-of-function variants in human INHBE are associated with favorable fat distribution and reduced risk of type 2 diabetes, highlighting its clinical relevance (4). Expression of INHBE is upregulated by ATF4 during endoplasmic reticulum stress, linking it to nutrient sensing and adaptive metabolic responses (5). These findings underscore the potential of recombinant Activin E as a therapeutic target for metabolic disorders such as nonalcoholic fatty liver disease (NAFLD) and obesity (5).

References

  1. Morita, M. Hashimoto, O. (2019) Molecular Biology Reports 46:1603. 
  2.  Adam, R.C. et al. (2023) Proc Natl Acad Sci U S A 120:e2309967120 
  3.  Sugiyama, M. et al. (2018) Plos one 13:e0194798.
  4.  Deaton, A.M. et. al. (2022) Nat Commun. 13: 4319. 
  5. Park, S.Y. et al. (2025) Exp Mol Med 57:466.

Long Name

Inhibin Subunit Beta E

Alternate Names

Inhibin Beta E, MGC4638

Entrez Gene IDs

83729 (Human); 16326 (Mouse)

Gene Symbol

INHBE

UniProt

P58166.1

Additional INHBE Products

Product Documents for Recombinant Human Activin E Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Activin E Protein, CF

For research use only

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