Recombinant Human Active MEK2 Kinase His-tag Protein, CF New

Name: Recombinant Human Active MEK2 Kinase His-tag Protein, CF
Brand: R&D Systems
SKU: 11825-ME

R&D Systems, part of Bio-Techne | Catalog # 11825-ME

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Product Datasheet / COA

Key Product Details

Source:

HEK293

View Full Technical Details for Recombinant Human Active MEK2 Kinase His-tag Protein, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Human Active MEK2 Kinase His-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human MEK2 protein
Leu2-Val400, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Protein identity confirmed by mass spectrometry

Predicted Molecular Mass

45 kDa

SDS-PAGE

45 kDa

Activity

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >7 pmol/min/μg, as measured under the described conditions.

Scientific Data Examples for Recombinant Human Active MEK2 Kinase His-tag Protein, CF

	Recombinant Human Active MEK2 Kinase His-tag Enzyme Activity. Recombinant Human Active MEK2 Kinase His-tag (Catalog # 11825-ME) is measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (DY996).
	Recombinant Human Active MEK2 Kinase His-tag SDS-PAGE. 2 μg/lane of Recombinant Human Active MEK2 Kinase His-tag (Catalog # 11825-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-47 kDa, under reducing conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11825-ME

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MEK2

MEK2 (MAPK/ERK kinase 2), also referred to as dual specificity mitogen-activated protein kinase kinase 2 (MAP2K2, MAPKK2) and ERK activator kinase 2 (ERK kinase 2), is a widely expressed cytoplasmic kinase from the protein kinase MAP kinase family. MEK1 and MEK2 are dual specificity enzymes that can phosphorylate threonine and tyrosine residues in hundreds of substrates within the RAS-MAPK-ERK pathway (1-3). Like, MEK1, MEK2 contains an N-terminus with an ERK binding domain, nuclear export sequence and a regulatory domain, a kinase domain with a P loop, ATP-binding site, activation loop, and proline rich domain, and a C-terminal region with a docking domain for binding to upstream kinases (3, 4). Although MEK1 and MEK2 are highly conserved and 86% identical in their catalytic domain, they diverge in their N-terminal and proline rich domains which contribute to distinct biological function through differing regulation (2, 3, 5, 6). Activation of both MEKs occurs through phosphorylation by upstream kinases, such as RAF kinases, which causes conformational changes in the kinase that allow it to phosphorylate ERK1/2 with high substrate specificity (3). Pathogenic variants in the MEK2 gene cause a gain of function in the RAS-MAPK pathway and result in a rare genetic condition called cardiofaciocutaneous syndrome type 4 (CFC4) (7). In addition, as a critical component of the MAP kinase signal transduction pathway, MEK2 participates in signaling cascades that transmit a variety of extra- and intracellular signals to mediate diverse biological functions such as cell growth, proliferation, survival, and differentiation (2, 3) frequently found to be dysregulated in cancers such as colorectal, pancreatic, endometrial, thyroid, and lung that involve BRAF and KRAS mutations (3). Significant research has been performed to discover targeted and combination small molecule and antibody inhibitors of MEK proteins as research tools to facilitate a better understanding of ERK biology and also to target MEK proteins for pharmacological intervention in cancer therapy and other diseases associated with MEK and MAP kinase pathway dysfunction (2, 3, 8-10).

Item	Value
References	Zheng, C.F. and K.L. Guan. (1993) J. Biol. Chem. 268:11435. Fremin, C. and S. Meloche. (2010) J. Hematol. Oncol. 3:8. Chan, W.Y. et al. (2026) Lancet 407:1639. Ohren, J.F. et al. (2004) Nat. Struct. Mol. Biol. 12:1192. Wu, X. et al. (1996) J. Biol. Chem. 271:3265. Xu, S. et al. (1997) Mol. Endocrinol. 11:1618. Scorrano, G. et al. (2023) Genes (Basel) 14:2111. Sebolt-Leopold, J.S. et al. (1999) Nature Med. 5:810. Bahar, M.E. et al. (2023) Signal Transduct. Target Ther. 8:455. Gouda, M.A. et al. (2023) ESMO Open 8:100788.
Long Name	Mitogen-activated Protein Kinase Kinase 2
Entrez Gene IDs	5605 (Human); 26396 (Mouse)
Alternate Names	ERK activator kinase 2, MAP2K2, MAPKK 2, MEK 2, MEK2, MEK2EC 2.7.12.2, MKK2, MKK2FLJ26075, PRKMK2MAPKK2, mitogen-activated protein kinase kinase 2, p45

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Product Documents for Recombinant Human Active MEK2 Kinase His-tag Protein, CF

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