Recombinant Human Active ERK1 Protein, CF

Catalog # 1879-KS | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

NM_002746

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human ERK1 protein

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

N-terminal Sequence Analysis

Activated by active MEK1 in vitro

Predicted Molecular Mass

44 kDa

Activity

The specific activity of ERK1 was determined to be 42 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 382 nmol/min/mg as per radiometric assay.

Scientific Data Images for Recombinant Human Active ERK1 Protein, CF

Recombinant Human Active ERK1 Protein SDS-PAGE

Recombinant Human Active ERK1 Protein SDS-PAGE.

The approximate molecular weight is 44 kDa and the purity is > 80%.

Formulation, Preparation and Storage

1879-KS
Formulation Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

References

  1. Boulton, T.G. et al. (1991) Biochemistry 30:278.

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Gene Symbol

MAPK3

UniProt

NM_002746

Product Documents for Recombinant Human Active ERK1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Active ERK1 Protein, CF

For research use only

Citations for Recombinant Human Active ERK1 Protein, CF (2)

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FAQs for Recombinant Human Active ERK1 Protein, CF

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  • Q: What is the activation protocol for this enzyme?

    A: This enzyme is supplied in its activated form, so no activation step is required.

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