Recombinant Human Active ERK1 Protein, CF
Catalog # 1879-KS | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
E. coli-derived human ERK1 protein
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Activated by active MEK1 in vitro
Predicted Molecular Mass
44 kDa
Activity
The specific activity of ERK1 was determined to be 42 nmol/min/mg using a myelin basic protein (MBP) substrate and was equivalent to 382 nmol/min/mg as per radiometric assay.
Scientific Data Images for Recombinant Human Active ERK1 Protein, CF
Recombinant Human Active ERK1 Protein SDS-PAGE.
The approximate molecular weight is 44 kDa and the purity is > 80%.Formulation, Preparation and Storage
1879-KS
Formulation | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF and 25% glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | This product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: ERK1
Product Documents for Recombinant Human Active ERK1 Protein, CF
Product Specific Notices for Recombinant Human Active ERK1 Protein, CF
For research use only
Citations for Recombinant Human Active ERK1 Protein, CF (2)
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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Active ERK1 Protein, CF include:
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Species: Human
Sample Types: Protein
Applications: BioassayY Fan et al. (2022-02-22), ERK1/2 inhibits Cullin 3/SPOP-mediated PrLZ ubiquitination and degradation to modulate prostate cancer progression Cell Death and Differentiation, 2022-00(0).
PMID: 35194188 -
Sample Types: N/A
Applications: Enzyme Assay(2011-08-11), Phosphatase-coupled universal kinase assay and kinetics for first-order-rate coupling reaction. PLoS ONE, 2011-06(8):e23172.
PMID: 21853082
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