Recombinant Human A4GNT Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8960-GT

R&D Systems, part of Bio-Techne
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8960-GT-020
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human A4GNT Protein (8960-GT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession #

Q9UNA3

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT protein
Leu25-Lys340, with C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

38 kDa

SDS-PAGE

39-49 kDa, reducing conditions

Activity

Measured by its ability to transfer GlcNAc from UDP-GlcNAc to galactose.
The specific activity is >400 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8960-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT

Alpha-1,4-N-acetylglucosaminyltransferase A4GNT is a type II Golgi resident membrane glycosyltransferase, but shows no significant sequence similarity with any other glycosyltransferases. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans and forms a unique glycan,
GlcNAc alpha1-->4Gal beta-->R on mucin (1). Alpha-1,4-GlcNAc-capped mucin-type O-glycan inhibits cholesterol alpha-glucosyltransferase from Helicobacter pylori (Hp) and suppresses Hp growth (2). Hp infection is widespread and thought to be the causes of the development of gastric lesions including gastritis, intestinal metaplasia, and gastric carcinoma (3). The enzymatic activity of the recombinant protein was determined using a phosphatase-coupled assay (4).

References

  1. Nakayama, J. et al. (1999) Proc. Natl. Acad. Sci. USA 96: 8991
  2. Lee, H. et al. (2008) Glycobiology 18:549.
  3. Ferreira, B. et al. (2006) J. Histochem. Cytochem. 54: 585.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

alpha4GnT

Entrez Gene IDs

51146 (Human); 333424 (Mouse); 685758 (Rat)

Gene Symbol

A4GNT

UniProt

Q9UNA3

Additional Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Products

Product Documents for Recombinant Human A4GNT Protein, CF

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Product Specific Notices for Recombinant Human A4GNT Protein, CF

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