Recombinant E. coli Ecotin Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1328-PI
Key Product Details
- R&D Systems E. coli-derived Recombinant E. coli Ecotin Protein (1328-PI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Source
E. coli-derived e. coli Ecotin protein
Ala21-Arg162, with a C-terminal 6-His tag
Ala21-Arg162, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
17 kDa
SDS-PAGE
18 kDa, reducing conditions
Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <1.0 nM, as measured under the described conditions.
The IC50 value is <1.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1328-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 350 mM NaCl, pH 8.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Ecotin
Ecotin is a potent and general inhibitor of serine proteases with diverse substrate specificities (1). It strongly inhibits trypsin, chymotrypsin, elastase, factor Xa, plasma kallikrein, u-plasminogen activator, granzyme B, and factor XIIa. Immobilized Ecotin has been used to affinity-purify recombinant trypsinogen, indicating that it may also be used to purify additional serine protease zymogens (2). Compared to other serine protease inhibitors such as members of the serpin family, the reactive site of Ecotin is Met104 (P1) (3). Ecotin is synthesized as a 162 amino acid precursor with a 20 amino acid signal peptide necessary to direct it to the periplasmic space. The mature protein (residues 21‑162) is expressed and purified.
References
- McGrath, M.E. et al. (1995) Protein Sci. 4:141.
- Lengyel, Z. et al. (1998) Protein Expre. Purif. 12:291.
- McGrath, M.E. et al. (1991) J. Biol. Chem. 28:6620.
Gene Symbol
eco
UniProt
Additional Ecotin Products
Product Documents for Recombinant E. coli Ecotin Protein, CF
Product Specific Notices for Recombinant E. coli Ecotin Protein, CF
For research use only
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