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Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

Catalog # 10306-IR | R&D Systems, Inc. a Bio-Techne Brand
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10306-IR-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-23R protein
Cynomolgus Monkey IL-23R
(Gly24-Asp353)
Accession # XP_005543141
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

65 kDa

SDS-PAGE

91-102 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50-250 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein Binding Activity

When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 µg/mL, 100 µL/well, the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50-250 ng/mL.
Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 91-102 kDa and 180-200 kDa, respectively.

Formulation, Preparation and Storage

10306-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human TH0, TH1 as well as several NK cell lines. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23 R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23 R, respectively.

References

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and Frucht, D.M. (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
  6. Schroder, J. et al. (2015) J. Biol. Chem. 290:359.

Long Name

Interleukin 23 Receptor

Alternate Names

IL-23 R, IL23R

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Gene Symbol

IL23R

UniProt

Product Documents for Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

For research use only

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