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Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Catalog # 10226-LR | R&D Systems, Inc. a Bio-Techne Brand
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10226-LR-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey LAIR1 protein
Cynomolgus Monkey LAIR1
(Gln22-His163)
Accession # XP_015297320.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln22, deduced from deblocked sequence at Glu23

Predicted Molecular Mass

42 kDa

SDS-PAGE

58-64 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Cynomolgus Monkey LAIR1 Fc Chimera binds with an ED50 of 1‑6 ng/mL.

Scientific Data Images for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Recombinant Cynomolgus LAIR1 Fc Chimera Protein Binding Activity

Recombinant Cynomolgus LAIR1 Fc Chimera Protein Binding Activity

When Bovine Collagen I is coated at 10 μg/mL, 100 μL/well, Recombinant Cynomolgus LAIR1 Fc Chimera (Catalog # 10226-LR) binds with an ED50 of 1-6 ng/mL.
Recombinant Cynomolgus LAIR1 Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus LAIR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus LAIR1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

10226-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). Cynomolgus LAIR-1 cDNA encodes a 296 amino acid (aa) polypeptide precursor. Based on the similarity with human LAIR1, it is likely to be a type I transmembrane (TM) protein containing a 21 aa signal sequence, a 144 aa extracellular domain (ECD), a 21 aa TM domain and a 110 aa cytoplasmic domain. Within ECD cynomolgus LAIR-1 shares 82% aa identity with human LAIR-1. LAIR-1 ECD includes one C2-type Ig-like domain. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6).

References

  1. Meyaard, L.(2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Product Documents for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

For research use only

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