Recombinant Canine IL-2 (Cys147Ser) Protein

Name: Recombinant Canine IL-2 (Cys147Ser) Protein
Brand: R&D Systems
SKU: 1815-CL
Rating: 4 (1 reviews)

Catalog # 1815-CL | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (2)

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Key Product Details

Source:	E. coli
Accession #:	Q29416
Applications:	Bioactivity

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Product Specifications for Recombinant Canine IL-2 (Cys147Ser) Protein

Source	E. coli-derived canine IL-2 protein Ala21-Thr155 (Cys147Ser), with and without an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met & Ala21
Predicted Molecular Mass	15.6 kDa
Activity	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.15‑0.8 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1815-CL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1815-CL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes (2). The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits (3). IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals (4). In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9 (6). At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively (7).

Item	Value
References	Morgan, D.A. et al. (1976) Science 193:1007. Smith, K.A. et al. (1988) Science 240:1169. Taniguchi, T. and Y. Minami (1993) Cell 73:5. Giri, J. et al. (1994) EMBO J. 13:2822. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1. Dunham, S.P. et al. (1995) DNA Seq. 5:177.
Long Name	Interleukin 2
Entrez Gene IDs	3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names	Aldesleukin, IL-2, IL-2lymphokine, IL2, Proleukin, T cell growth factor, T-cell growth factor, TCGF, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion

Citations for Recombinant Canine IL-2 (Cys147Ser) Protein (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Canine IL-2 (Cys147Ser) Protein include:

Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

BM Scorza et al. (2022-04-13), Role of NK-Like CD8+ T Cells during Asymptomatic Borrelia burgdorferi Infection Infection and Immunity, 2022-00(0):e0055521.
PMID: 35416707
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay

Haurigot V et al. (2010-04-27), Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs. Mol. Ther., 2010-018(7):1318-29.
PMID: 20424599

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Recombinant Canine IL-2 (Cys147Ser) Protein

Name: Anonymous

Application: CellProlif

Verified Customer | Posted 09/07/2018

Recombinant Canine IL-2 (Cys147Ser) Protein

Catalog # 1815-CL | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Canine IL-2 (Cys147Ser) Protein

Formulation, Preparation and Storage

Carrier Free

1815-CL

Carrier

1815-CL/CF

Carrier Free

Reconstitution Calculator

Background: IL-2

Citations for Recombinant Canine IL-2 (Cys147Ser) Protein (2)

Customer Reviews for Recombinant Canine IL-2 (Cys147Ser) Protein (1)

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Recombinant Canine IL-2 (Cys147Ser) Protein

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