Recombinant Human Rad51L1 His Protein

Name: Recombinant Human Rad51L1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23395

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23395

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Rad51L1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-350 of Human Rad51L1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

40.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Rad51L1 His Protein

SDS-PAGE: Recombinant Human Rad51L1 His Protein [NBP2-23395]

SDS-Page: Rad51L1 Protein [NBP2-23395]

Formulation, Preparation and Storage

NBP2-23395

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad51L1

RAD51B is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. Recombinant human RAD51B protein, fused to His-tag at N-terminus, was expressed in E.coli .

Alternate Names

DNA repair protein RAD51 homolog 2, MGC34245, R51H2REC2hREC2, RAD51 (S. cerevisiae)-like 1, RAD51 homolog B, RAD51B, RAD51-like 1 (S. cerevisiae), RAD51-like protein 1, RecA-like protein, recombination repair protein

Gene Symbol

RAD51B

Additional Rad51L1 Products

Product Documents for Recombinant Human Rad51L1 His Protein

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COA

Product Specific Notices for Recombinant Human Rad51L1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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