Recombinant Human RACK1/GNB2L1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78874

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all RACK1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-317 of Human RACK1/GNB2L1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA GYTDNLVRVW QVTIGTR

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

37.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human RACK1/GNB2L1 His Protein

SDS-PAGE: Recombinant Human RACK1/GNB2L1 His Protein [NBP1-78874]

SDS-PAGE: Recombinant Human RACK1/GNB2L1 His Protein [NBP1-78874]

SDS-Page: Recombinant Human RACK1/GNB2L1 His Protein [NBP1-78874] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-78874
Formulation 20 mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.2 M NaCl, 5mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RACK1

GNB2L1 was originally identified as an anchoring protein for protein kinase C beta (PKCbeta), which it stabilises in the active state and anchors to membranes or functional sites. However, evidence has accumulated to support a central role of GNB2L1 in critical biological responses. In addition to binding specifically to the active form of PKCbeta isoforms, GNB2L1 also interacts with several other important signaling proteins including the Src kinase family, integrin beta1, integrin beta2, integrin beta3 and integrin beta5, beta-spectrin and dynamin, RasGAP, the androgen receptor, insulin-like growth factor 1 receptor (IGF-1r), Epstein-Barr virus trans-activator protein BZLF1, p73 and pRB. GNB2L1 is ubiquitously expressed in the tissues of higher mammals and humans including brain, liver, and spleen. Recombinant human GNB2L1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Receptor for Activated Protein Kinase C 1

Alternate Names

GNB2L1

Gene Symbol

RACK1

Additional RACK1 Products

Product Documents for Recombinant Human RACK1/GNB2L1 His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human RACK1/GNB2L1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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