During my master thesis, I am working on a large scale ribosome affinity purification in Trypanosoma brucei. Unfortunately, we have some problems with the present antibody (IgG against protein A, extracted from chicken). Therefore, we are searching a better and more efficient alternative. Ideally it is extracted from guinea pig, rat, mouse or rabbit. Searching suitable antibodies, I found you have some in your range of products. Since we need to work large scale and will need a bigger amount of antibodies the next months, we would like to get the possibility to test the new antibodies first. Therefore I would like to ask for an aliquot for testing. You would really help me finding an good alternative!

Name: Recombinant Protein A His (C-Term) Cys (C-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35014

We unfortunately cannot offer testing samples of our antibodies. I am sorry for any inconvenience. We do have two antibodies to Protein A that meet your host requirements. We fully guarantee our antibodies for all listed species and applications. For any applications and/or species not listed, we offer our Innovators Reward Program. In exchange for a review of your experiment using our product in an untested application or species, we will issue you a credit for the purchase price of the antibody.

Recombinant Protein A His (C-Term) Cys (C-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35014

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His (C-Term), Cys (C-Term)

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Protein A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 307 amino acids corresponding to Protein A with a 6x His-tag and Cys on the C-terminus Source: E. coli

Uniprot ID: P02976

Amino Acid Sequence: MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Protein A as determined by LAL method.

Predicted Molecular Mass

34.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to

Specificity

Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Protein A His (C-Term) Cys (C-Term) Protein

SDS-PAGE: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]

SDS-Page: Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]

Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]

HPLC: Recombinant Protein A His (C-Term) Cys (C-Term) Protein [NBP2-35014]

HPLC: Recombinant Protein A Protein [NBP2-35014] - Protein A Cys (C-Term) His (C-Term) Protein [NBP2-35014]

Formulation, Preparation and Storage

NBP2-35014

Formulation	Lyophilized from additive free solution.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Reconstitute with distilled water or saline.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Protein A

Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.

Alternate Names

PROA, Protein-A

Gene Symbol

SAOUHSC_00069

Additional Protein A Products

Product Documents for Recombinant Protein A His (C-Term) Cys (C-Term) Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Protein A His (C-Term) Cys (C-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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