Recombinant Human PP2C gamma/PPM1G His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18399

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all PP2C gamma Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 317-546 of Human PP2C gamma/PPM1G

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PP2C gamma/PPM1G His Protein

SDS-PAGE: Recombinant Human PP2C gamma/PPM1G His Protein [NBC1-18399]

SDS-PAGE: Recombinant Human PP2C gamma/PPM1G His Protein [NBC1-18399]

SDS-Page: PPM1G Protein [NBC1-18399] - PPM1G, 27 kDa (250 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18399
Formulation 25mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 1 mM EDTA, 2 mM beta-mercaptoethanol, 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PP2C gamma

PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Recombinant human PPM1G, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Gene Symbol

PPM1G

Additional PP2C gamma Products

Product Documents for Recombinant Human PP2C gamma/PPM1G His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human PP2C gamma/PPM1G His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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