Recombinant Human NUDT21 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51010

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NUDT21 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-227 of Human NUDT21

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NUDT21 His Protein

SDS-PAGE: Recombinant Human NUDT21 His Protein [NBP1-51010]

SDS-PAGE: Recombinant Human NUDT21 His Protein [NBP1-51010]

SDS-Page: Recombinant Human NUDT21 Protein [NBP1-51010] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-51010
Formulation 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 2 mM DTT, 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NUDT21

NUDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing. Recombinant human NUDT21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CFIm25, CFIM25CPSF 25 kDa subunit, cleavage and polyadenylation specific factor 5, 25 kD subunit, cleavage and polyadenylation specific factor 5, 25 kDa, Cleavage and polyadenylation specificity factor 25 kDa subunit, cleavage and polyadenylation specificity factor subunit 5, CPSF25, CPSF5DKFZp686H1588, Nucleoside diphosphate-linked moiety X motif 21, nudix (nucleoside diphosphate linked moiety X)-type motif 21, Nudix motif 21, pre-mRNA cleavage factor Im (25kD), Pre-mRNA cleavage factor Im 25 kDa subunit, pre-mRNA cleavage factor Im, 25kD subunit

Gene Symbol

NUDT21

Additional NUDT21 Products

Product Documents for Recombinant Human NUDT21 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human NUDT21 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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