Recombinant Human NEU-1/Sialidase-1 His Protein

Name: Recombinant Human NEU-1/Sialidase-1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23471

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23471

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Scientific Data
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Background
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all NEU-1/Sialidase-1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 48-415 of Human NEU-1/Sialidase-1

Source: E.coli

Amino Acid Sequence: ENDFG LVQPLVTMEQ LLWVSGRQIG SVDTFRIPLI TATPRGTLLA FAEARKMSSS DEGAKFIALR RSMDQGSTWS PTAFIVNDGD VPDGLNLGAV VSDVETGVVF LFYSLCAHKA GCQVASTMLV WSKDDGVSWS TPRNLSLDIG TEVFAPGPGS GIQKQREPRK GRLIVCGHGT LERDGVFCLL SDDHGASWRY GSGVSGIPYG QPKQENDFNP DECQPYELPD GSVVINARNQ NNYHCHCRIV LRSYDACDTL RPRDVTFDPE LVDPVVAAGA VVTSSGIVFF SNPAHPEFRV NLTLRWSFSN GTSWRKETVQ LWPGPSGYSS LATLEGSMDG EEQAPQLYVL YEKGRNHYTE SISVAKISVY GTL

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human NEU-1/Sialidase-1 His Protein

SDS-PAGE: Recombinant Human NEU-1/Sialidase-1 His Protein [NBP2-23471]

SDS-Page: Recombinant Human NEU-1/Sialidase-1 Protein [NBP2-23471]

Formulation, Preparation and Storage

NBP2-23471

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEU-1/Sialidase-1

NEU1 is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red -myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity. Recombinant human NEU1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

N-acetyl-alpha-Neuraminidase 1

Alternate Names

NEU1, Sialidase-1

Gene Symbol

NEU1

Additional NEU-1/Sialidase-1 Products

Product Documents for Recombinant Human NEU-1/Sialidase-1 His Protein

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COA

Product Specific Notices for Recombinant Human NEU-1/Sialidase-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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