Recombinant Human MLC1SA His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72448

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NBP1-72448-0.5mg
NBP1-72448-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all MLC1SA Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-208 of Human MLC1SA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPPKKDV PVKKPAGPSI SKPAAKPAAA GAPPAKTKAE PAVPQAPQKT QEPPVDLSKV VIEFNKDQLE EFKEAFELFD RVGDGKILYS QCGDVMRALG QNPTNAEVLK VLGNPKSDEL KSRRVDFETF LPMLQAVAKN RGQGTYEDYL EGFRVFDKEG NGKVMGAELR HVLTTLGEKM TEEEVETVLA GHEDSNGCIN YEAFLKHILS V

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MLC1SA His Protein

SDS-PAGE: Recombinant Human MLC1SA His Protein [NBP1-72448]

SDS-PAGE: Recombinant Human MLC1SA His Protein [NBP1-72448]

SDS-Page: Recombinant Human MLC1SA Protein [NBP1-72448] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72448
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLC1SA

MYL6B is a heavy chain regulator found in smooth muscle and non-muscle Myosin complexes. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain by Myosin light chain kinase. MYL6B does not bind calcium during contraction. It is primarily found in a hexamer consisting of four light chains and two heavy chains. It most commonly interacts with Myosin Va, an Actin based motor that can move in large steps. MYL6B is expressed in most tissues with neurons and smooth muscle tissue having the highest expression. Recombinant human MYL6B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

MLC1sa, MLC1SAmyosin light chain 1, slow-twitch muscle A isoform, myosin alkali light chain 1 slow a, myosin light chain 1 slow a, Myosin light chain 1 slow-twitch muscle A isoform, myosin light chain 6B, myosin, light chain 6B, alkali, smooth muscle and non-muscle, myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle, smooth muscle and non-muscle myosin alkali light chain 6B, Smooth muscle and nonmuscle myosin light chain alkali 6B

Gene Symbol

MYL6B

Additional MLC1SA Products

Product Documents for Recombinant Human MLC1SA His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MLC1SA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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