Recombinant Human MDH1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45315

Novus Biologicals, part of Bio-Techne
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NBP1-45315-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all MDH1 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-334 of Human MDH1

Source: E.coli

Amino Acid Sequence: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MDH1 His Protein

SDS-PAGE: Recombinant Human MDH1 His Protein [NBP1-45315]

SDS-PAGE: Recombinant Human MDH1 His Protein [NBP1-45315]

SDS-Page: Recombinant Human MDH1 His Protein [NBP1-45315] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-45315
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDH1

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Gene Symbol

MDH1

Additional MDH1 Products

Product Documents for Recombinant Human MDH1 His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human MDH1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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