Recombinant GST Epitope Tag His Protein

Name: Recombinant GST Epitope Tag His Protein
Brand: Novus Biologicals
SKU: NBP1-30259

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30259

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all GST Epitope Tag Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Schistosoma japonicum GST Epitope Tag

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant GST Epitope Tag His Protein

SDS-PAGE: Recombinant GST Epitope Tag His Protein [NBP1-30259]

SDS-Page: Recombinant Parasite GST Epitope Tag His Protein [NBP1-30259] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-30259

Formulation	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GST Epitope Tag

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E.coli source. Recombinant human GST, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

EC 2.5.1.18, glutathione S-alkyltransferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase M1, glutathione S-transferase mu 1, GST class-mu 1, GST HB subunit 4, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, HB subunit 4, MGC26563, MU, MU-1, S-(hydroxyalkyl)glutathione lyase

Gene Symbol

GSTM1

Additional GST Epitope Tag Products

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COA

Product Specific Notices for Recombinant GST Epitope Tag His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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