Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

Name: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein
Brand: Novus Biologicals
SKU: L-1104-NB

Novus Biologicals, part of Bio-Techne | Catalog # L-1104-NB

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Unconjugated

Applications

Bioactivity, Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

View all Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Proteins and Enzymes »

Product Specifications

Description

A biologically active protein corresponding to Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4.

Source: Griffonia (Bandeiraea) simplicifolia seeds

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. This “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues.

For reconstituted liquid, storage at 4 C with 0.08% sodium azide can be used. For preserving solutions stored at 4C, 0.08% sodium azide can be used. Specific for galactose. Inhibitors: 500 mM galactose or 100 mM raffinose.

Purity

Affinity purified

Predicted Molecular Mass

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues.

Application Notes

Protein / Peptide Type

Native Protein

Formulation, Preparation and Storage

L-1104-NB

Formulation	Lyophilized from water
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM Ca2+ is recommended.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C.

Background: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4

GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B , with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for alpha-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for alpha-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.

GSL I-B4 isolectin contains only the B subunits. It is a useful marker for endothelial cells from non-primates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons.

Inhibiting Sugar: 500 mM galactose or 100 mM raffinose

Additional Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Products

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COA

Product Specific Notices for Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 Native Protein

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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