Recombinant Human FGF-12 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61375
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 181 amino acids corresponding to FGF-12 Source: E. coli
Amino Acid Sequence: MESKEPQLKG IVTRLFSQQG YFLQMHPDGT IDGTKDENSD YTLFNLIPVG LRVVAIQGVK ASLYVAMNGE GYLYSSDVFT PECKFKESVF ENYYVIYSST LYRQQESGRA WFLGLNKEGQ IMKGNRVKKT KPSSHFVPKP IEVCMYREQS LHEIGEKQGR SRKSSGTPTM NGGKVVNQDS T
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of FGF-12 as determined by LAL method.
Predicted Molecular Mass
20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
The biological activity was determined by its binding ability in a functional ELISA. Immobilized rHuFGF R4/Fc Chimera at 5 ug/mL (100 uL/well) can bind rHuFGF-12 with a linear range of 1.6-100 ng/mL.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-61375
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 1 mM DTT. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: FGF-12
FGF-12 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. FGF-11, -12, -13, -14, originally termed FGF homologous factors (FHF) -3, -1, -2, and -4, respectively, form a subgroup within the FGF family. Human FGF-12/FHF-1 is synthesized as a 243 aa protein. It lacks a typical signal sequence and is considered cytoplasmic. It also possess an N-terminal bipartite nuclear localization signal at aa 11-18 and 28-38.
Long Name
Fibroblast Growth Factor 12
Alternate Names
FGF12, FHF1
Gene Symbol
FGF12
Additional FGF-12 Products
Product Documents for Recombinant Human FGF-12 Protein
Product Specific Notices for Recombinant Human FGF-12 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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