Recombinant Human EHF His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23029

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NBP2-23029
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EHF Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-300 of Human EHF

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMILEGGG VMNLNPGNNL LHQPPAWTDS YSTCNVSSGF FGGQWHEIHP QYWTKYQVWE WLQHLLDTNQ LDANCIPFQE FDINGEHLCS MSLQEFTRAA GTAGQLLYSN LQHLKWNGQC SSDLFQSTHN VIVKTEQTEP SIMNTWKDEN YLYDTNYGST VDLLDSKTFC RAQISMTTTS HLPVAESPDM KKEQDPPAKC HTKKHNPRGT HLWEFIRDIL LNPDKNPGLI KWEDRSEGVF RFLKSEAVAQ LWGKKKNNSS MTYEKLSRAM RYYYKREILE RVDGRRLVYK FGKNARGWRE NEN

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

37.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EHF His Protein

SDS-PAGE: Recombinant Human EHF His Protein [NBP2-23029]

SDS-PAGE: Recombinant Human EHF His Protein [NBP2-23029]

SDS-Page: EHF Protein [NBP2-23029]

Formulation, Preparation and Storage

NBP2-23029
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EHF

ETS homologous factor isoform 2, also known as EHF, is a distinct member of the ESE subfamily of Ets transcription factors. Ets factors constitute one important class of transcriptional regulators that play critical roles in hema-topoiesis, angiogenesis, organogenesis, oncogenesis and specification of neuronal connectivity. It is exclusively expressed in a subset of epithelial cells, with highest expression detected in glandular epithelium of the prostate, pancreas, salivary gland and trachea. EHF transactivates the c-Met promoter via three high affinity binding sites, which suggests that EHF may contribute to branching morphogenesis. Recombinant human EHF protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

Epithelium-specific Ets transcription factor 3, ESE-3, ESE3 transcription factor, ESE3B, ESE3hEHF, ESEJepithelium-specific ets factor 3, ETS domain-containing transcription factor, ets homologous factor

Gene Symbol

EHF

Additional EHF Products

Product Documents for Recombinant Human EHF His Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human EHF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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