Recombinant eGFP Protein

Name: Recombinant eGFP Protein
Brand: Novus Biologicals
SKU: NBP2-34923

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34923

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P42212

Conjugate

Unconjugated

Applications

SDS-PAGE, Western Blot

View all eGFP Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 239 amino acids corresponding to eGFP Source: E. coli

Uniprot ID: P42212

Amino Acid Sequence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of eGFP as determined by LAL method.

Predicted Molecular Mass

26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Use in WB reported in scientific literature (PMID:10.1007/s00299-021-02700-w)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant eGFP Protein

SDS-PAGE: Recombinant eGFP Protein [NBP2-34923]

SDS-Page: Human GFP Protein [NBP2-34923] - GFP Protein [NBP2-34923]

Formulation, Preparation and Storage

NBP2-34923

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: eGFP

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

Alternate Names

Enhanced Green Fluorescent Protein, GFP, Green Fluorescent Protein

UniProt