Recombinant Virus Dengue Virus 1 VLP Protein

Name: Recombinant Virus Dengue Virus 1 VLP Protein
Brand: Novus Biologicals
SKU: NBP3-14811

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14811

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Product Datasheet / COA

Discontinued Product

NBP3-14811 has been discontinued. View all Dengue Virus 1 VLP products.

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

HEK293

Conjugate

Unconjugated

Applications

Microscopy, Western Blot

View all Dengue Virus 1 VLP Proteins and Enzymes »

Product Specifications

Description

Recombinant Dengue Virus serotype 1 virus-like particle recombinant antigen consisting of Envelope, pre-Membrane and Membrane protein (NCBI Accession Number: ABO45246.1). The C-terminal 20% of the dengue Envelope protein was replaced by the corresponding Japanese Encephalitis Virus sequence (all internal/transmembrane).

Storage: Avoid excessive mixing or shocking to prevent aggregation. Long term storage above -80C may result in aggregate formation.

Concentration: 0.1-0.6mg/ml

Purity

>95% pure by SDS-PAGE

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Virus Dengue Virus 1 VLP Protein

Western Blot: Recombinant Virus Dengue Virus 1 VLP Protein [NBP3-14811]

Western Blot: Dengue Virus 1 VLP Recombinant Protein Antigen [NBP3-14811] - Non-reducing SDS-PAGE gel showing purified Dengue virus serotype 1 virus-like particles; envelope protein (E) at approx. 55kDa, and prM protein running at approx. 18kDa.

Electron Microscopy: Dengue Virus 1 VLP Recombinant Protein Antigen [NBP3-14811] - Negative staining electron microscopy of DENV1 virus-like particles (VLPs).

Formulation, Preparation and Storage

NBP3-14811

Preparation Method	Recombinant Dengue Virus serotype 1 virus-like particle recombinant antigen consisting of Envelope, pre-Membrane and Membrane protein produced in HEK293 human cells. The C-terminal 20% of the dengue Envelope protein was replaced by the corresponding Japanese Encephalitis virus sequence. Tested for stability, concentrated and purified by a series of ultracentrifugation and chromatographical methods which result in VLPs of exceptional quality and purity.
Formulation	10mM sodium phosphate, 20mM sodium citrate, 154mM sodium chloride, pH7.4
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Dengue Virus 1 VLP

Dengue virus is a single -stranded, positive-sense RNA virus belonging to the Flavivirdae family and is the causative agent of Dengue fever (1-3). Other viruses in the same family include Japanese encephalitis virus, West Nile virus, and yellow fever (2,3). The Dengue virus is primarily transmitted through Aedes mosquito bites, with 100-400 million people infected annually and approximately 20,000 deaths worldwide per year (1,3). There are four known Dengue virus serotypes (DENV 1-4) that share 65% amino acid sequence similarity (1-2). The serotypes can be further classified into genotypes according to geographical distribution (2,3). The structure of the Dengue virus is ~50 nanometers (nm) in diameter, spherical in shape, and consists of an enveloped single-stranded RNA, an icosahedral nucleocapsid core, and a lipid bilayer (1-3). The Dengue virus genome is 11-kilobases (kb) long and has a 5' untranslated region (UTR), an open reading frame (ORF), and a 3' UTR (1,3). The ORF encodes a polyprotein for the translation of the three structural proteins and seven non-structural proteins (1-3). The structural proteins are the capsid (100 amino acids (aa), 12 kilodalton (kDa)), the pre-membrane (166 aa) or membrane (75 aa, 8.2-8.5 kDa), and envelope (495 aa, 53 kDa) (1-3). The nonstructural proteins are NS1 (350 aa, 45 kDa), NS2A (218 aa, 22 kDa), NS2B (130 aa, 14 kDa), NS3 (618 aa, 70 kDa), NS4A (150 aa, 16 kDa), NS4B (245-249 aa, 27 kDa), NS5 (900 aa, 104 kDa) (1-3).

Dengue virus entry into host cells occurs via receptor-mediated endocytosis by receptor molecules including the mannose receptor, heparan sulfate, glycosaminoglycans, and DC-SIGN (1,3). Following attachment, the virus is endocytosed in clathrin-coated vesicles (1,3). Following internalization, clathrin disassembles and endosomal processing occurs, allowing viral fusion, disassembly, and release of viral RNA (1,3). This release results in viral translation and replication, virus assembly and maturation, and eventual exocytosis of the mature virus (1,3). Infection can result in a wide range of clinical symptoms including mild disease such as Dengue fever which is characterized by fever, headache, joint pain, rash, and retro-orbital pain, or severe, life-threatening conditions like Dengue hemorrhagic fever or Dengue shock syndrome which involves vascular permeability and leakage (1-3). Host immune response against infection includes innate immune response via interferon secretion and pro-inflammatory cytokine production, as well as adaptive immune response involving cellular and humoral components like T cell activation and B-cell mediated antibody production (1-3). As far as treatment for Dengue virus infection, there no commercial antiviral agents, though some anti-pyretics and certain phenolic compounds do show promise in treating infection (1-3). However, Resveratol, an antiviral for other Flavivirus, has been shown to directly attack the Dengue virus genome (1). While more work needs to be done, there are some live-attenuated tetravalent Dengue virus vaccine candidates in clinal trials including DENVax and TV003/TV005 (1-3).

References

1. Nanaware N, Banerjee A, Mullick Bagchi S, Bagchi P, Mukherjee A. Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses. Viruses. 2021;13(10):1967. Published 2021 Sep 30. https://doi.org/10.3390/v13101967

2. Harapan H, Michie A, Sasmono RT, Imrie A. Dengue: A Minireview. Viruses. 2020;12(8):829. Published 2020 Jul 30. https://doi.org/10.3390/v12080829

3. Roy SK, Bhattacharjee S. Dengue virus: epidemiology, biology, and disease aetiology. Can J Microbiol. 2021;67(10):687-702. https://doi.org/10.1139/cjm-2020-0572

Alternate Names

polyprotein precursor

Product Documents for Recombinant Virus Dengue Virus 1 VLP Protein

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COA

Product Specific Notices for Recombinant Virus Dengue Virus 1 VLP Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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