Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

What is the endotoxin level of your recombinant human coagulation factor III/tissue factor protein (NBP2-22785)?

In regards to your inquiry about the endotoxin level of our recombinant human coagulation factor III/tissue factor protein (NBP2-22785), there is no exact value been provided. However, our protein products are within our regular cutoff for the reagent which is

Recombinant Human Coagulation Factor III/Tissue Factor His Protein

Name: Recombinant Human Coagulation Factor III/Tissue Factor His Protein
Brand: Novus Biologicals
SKU: NBP2-22785

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22785

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Coagulation Factor III/Tissue Factor Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 33-251 of Human Coagulation Factor III/Tissue Factor

Source: E.coli

Amino Acid Sequence: MSGTTNTVAA YNLTWKSTNF KTILEWEPKP VNQVYTVQIS TKSGDWKSKC FYTTDTECDL TDEIVKDVKQ TYLARVFSYP AGNVESTGSA GEPLYENSPE FTPYLETNLG QPTIQSFEQV GTKVNVTVED ERTLVRRNNT FLSLRDVFGK DLIYTLYYWK SSSSGKKTAK TNTNEFLIDV DKGENYCFSV QAVIPSRTVN RKSTDSPVEC MGQEKGEFRE LEHHHHHH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Coagulation Factor III/Tissue Factor His Protein

Recombinant Human Coagulation Factor III/Tissue Factor Protein [NBP2-22785] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-22785

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor III/Tissue Factor

Tissue factor, also known as F3 or CD142, is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Recombinant human F3 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Recombinant Human Coagulation Factor III/Tissue Factor His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Coagulation Factor III/Tissue Factor His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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